Structural insights into the functions of the FANCM-FAAP24 complex in DNA repair

	Structural insights into the functions of the FANCM-FAAP24 complex in DNA repair
	Yang, H; Zhang, TL; Tao, Y; Wang, F; Tong, L; Ding, JP
刊名	NUCLEIC ACIDS RESEARCH
	2013
卷号	41 期号:22 页码:10573-10583
通讯作者	Ding, JP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jpding@sibcb.ac.cn
英文摘要	Fanconi anemia (FA) is a genetically heterogeneous disorder associated with deficiencies in the FA complementation group network. FA complementation group M (FANCM) and FA-associated protein 24 kDa (FAAP24) form a stable complex to anchor the FA core complex to chromatin in repairing DNA interstrand crosslinks. Here, we report the first crystal structure of the C-terminal segment of FANCM in complex with FAAP24. The C-terminal segment of FANCM and FAAP24 both consist of a nuclease domain at the N-terminus and a tandem helix-hairpin-helix (HhH)(2) domain at the C-terminus. The FANCM-FAAP24 complex exhibits a similar architecture as that of ApXPF. However, the variations of several key residues and the electrostatic property at the active-site region render a catalytically inactive nuclease domain of FANCM, accounting for the lack of nuclease activity. We also show that the first HhH motif of FAAP24 is a potential binding site for DNA, which plays a critical role in targeting FANCM-FAAP24 to chromatin. These results reveal the mechanistic insights into the functions of FANCM-FAAP24 in DNA repair.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	ANEMIA CORE COMPLEX ; NUCLEOTIDE EXCISION-REPAIR ; MAINTAIN GENOME STABILITY ; CROSS-LINK REPAIR ; FANCONI-ANEMIA ; MONOUBIQUITINATED FANCD2 ; ENDONUCLEASE XPF-ERCC1 ; CRYSTAL-STRUCTURE ; BINDING-PROTEIN ; BLOOM-SYNDROME
收录类别	SCI
语种	英语
WOS记录号	WOS:000329874400053
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/466]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, H,Zhang, TL,Tao, Y,et al. Structural insights into the functions of the FANCM-FAAP24 complex in DNA repair[J]. NUCLEIC ACIDS RESEARCH,2013,41(22):10573-10583.
APA	Yang, H,Zhang, TL,Tao, Y,Wang, F,Tong, L,&Ding, JP.(2013).Structural insights into the functions of the FANCM-FAAP24 complex in DNA repair.NUCLEIC ACIDS RESEARCH,41(22),10573-10583.
MLA	Yang, H,et al."Structural insights into the functions of the FANCM-FAAP24 complex in DNA repair".NUCLEIC ACIDS RESEARCH 41.22(2013):10573-10583.