Drosophila Vps36 regulates Smo trafficking in Hedgehog signaling

	Drosophila Vps36 regulates Smo trafficking in Hedgehog signaling
	Yang, XF; Mao, FF; Lv, XD; Zhang, Z; Fu, L; Lu, Y; Wu, WQ; Zhou, ZC; Zhang, L; Zhao, Y
刊名	JOURNAL OF CELL SCIENCE
	2013
卷号	126 期号:18 页码:4230-4238
关键词	Drosophila Hedgehog Smoothened Trafficking Vps36
通讯作者	Zhao, Y (reprint author), Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,rayzhang@sibcb.ac.cn ; yunzhao@sibcb.ac.cn
英文摘要	The hedgehog (Hh) signaling pathway plays a very important role in metazoan development by controlling pattern formation. Malfunction of the Hh signaling pathway leads to numerous serious human diseases, including congenital disorders and cancers. The seven-transmembrane domain protein Smoothened (Smo) is a key transducer of the Hh signaling pathway, and mediates the graded Hh signal across the cell plasma membrane, thereby inducing the proper expression of downstream genes. Smo accumulation on the cell plasma membrane is regulated by its C-tail phosphorylation and the graded Hh signal. The inhibitory mechanism for Smo membrane accumulation in the absence of Hh, however, is still largely unknown. Here, we report that Vps36 of the ESCRT-II complex regulates Smo trafficking between the cytosol and plasma membrane by specifically recognizing the ubiquitin signal on Smo in the absence of Hh. Furthermore, in the absence of Hh, Smo is ubiquitylated on its cytoplasmic part, including its internal loops and C-tail. Taken together, our data suggest that the ESCRT-II complex, especially Vps36, has a special role in controlling Hh signaling by targeting the membrane protein Smo for its trafficking in the absence of Hh, thereby regulating Hh signaling activity.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	ENDOSOME-ASSOCIATED COMPLEX ; ESCRT-II ; MULTIVESICULAR-BODY ; UBIQUITIN RECOGNITION ; CUBITUS-INTERRUPTUS ; PATHWAY ACTIVATION ; EAP45-GLUE DOMAIN ; STRUCTURAL BASIS ; SORTING COMPLEX ; DOWN-REGULATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000324380700016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/458]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, XF,Mao, FF,Lv, XD,et al. Drosophila Vps36 regulates Smo trafficking in Hedgehog signaling[J]. JOURNAL OF CELL SCIENCE,2013,126(18):4230-4238.
APA	Yang, XF.,Mao, FF.,Lv, XD.,Zhang, Z.,Fu, L.,...&Zhao, Y.(2013).Drosophila Vps36 regulates Smo trafficking in Hedgehog signaling.JOURNAL OF CELL SCIENCE,126(18),4230-4238.
MLA	Yang, XF,et al."Drosophila Vps36 regulates Smo trafficking in Hedgehog signaling".JOURNAL OF CELL SCIENCE 126.18(2013):4230-4238.