Acetylcholinesterase function in apoptotic retina pigment epithelial cells induced by H2O2

	Acetylcholinesterase function in apoptotic retina pigment epithelial cells induced by H2O2
	Cai, L; Liao, HF; Zhang, XJ; Shao, Y; Xu, M; Yi, JL
刊名	INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
	2013
卷号	6 期号:6 页码:772-777
关键词	acetylcholinesterase retina pigment epithelial cells oxidative stress age-related macular degeneration
通讯作者	Yi, JL (reprint author), Jiangxi Res Inst Ophthalmol & Visual Sci, Nanchang 330006, Jiangxi, Peoples R China.,yj10791@126.com
英文摘要	AIM: To investigate the acetylcholinesterase (AChE) expression involved in retina pigment epithelial (RPE) apoptosis induced by higher concentrations H2O2. METHODS: The human retinal pigment epithelium cell line ARPE-19 was from ATCC (Rockville, MD). Cultured ARPE-19 cells were treated with H2O2 at 0, 250, 500, 1 000, 2 000 mu mol/L and cell viability was measured with MTT assay. AChE expression and DNA fragments were analyzed by immunocytochemistry, TUNEL and PARP-1 Western blotting. RESULTS: Immunofluorescence detected AChE exist in the normal human retinal tissue. When H2O2>500 mu mol/L, AChE expression showed an increase after 2h, and this concentration was selected for the present study. RPE cell was induced with 1 000 mu mol/L H2O2 for 2h, compared to the control group, cell activity decline detected by MTT, AChE and PARP-1 protein expression was significantly increased detected by Western blotting. AChE immunofluorescence staining was positive in RPE cell after H2O2 incubate 2h. In addition, pretreatment with 100 mu mol/L epigallocatechin gallate (EGCG), cell viability increased from 31.20%+/- 3.90% to 70.23%+/- 12.96%. CONCLUSION: AChE is weakly expressed in normal human RPE cells. Stimulation with H2O2 caused the stable increase of AChE expression in RPE cells, which may indicate that AChE may be an important role in AMD.
学科主题	Ophthalmology
类目[WOS]	Ophthalmology
关键词[WOS]	OXIDATIVE STRESS ; MACULAR DEGENERATION ; NECROSIS ; DAMAGE ; DEATH ; PREVALENCE ; MECHANISMS ; PATHWAY ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000328726300006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/396]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Cai, L,Liao, HF,Zhang, XJ,et al. Acetylcholinesterase function in apoptotic retina pigment epithelial cells induced by H2O2[J]. INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,2013,6(6):772-777.
APA	Cai, L,Liao, HF,Zhang, XJ,Shao, Y,Xu, M,&Yi, JL.(2013).Acetylcholinesterase function in apoptotic retina pigment epithelial cells induced by H2O2.INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,6(6),772-777.
MLA	Cai, L,et al."Acetylcholinesterase function in apoptotic retina pigment epithelial cells induced by H2O2".INTERNATIONAL JOURNAL OF OPHTHALMOLOGY 6.6(2013):772-777.