Purification and Identification of 72 kDa and 15 kDa Allergens from Broussonetia papyrifera Pollen

	Purification and Identification of 72 kDa and 15 kDa Allergens from Broussonetia papyrifera Pollen
	Chen, Z; Zhu, N; Chen, XD; Yang, Y; Li, YP; Wu, ZL; Chen, SX
刊名	IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY
	2013
卷号	12 期号:4 页码:312-320
关键词	Allergens Broussonetia papyrifera Chinese mulberry tree Pollen
通讯作者	Chen, XD (reprint author), Fudan Univ, Shanghai Huashan Hosp, 12 Urumqi Rd M, Shanghai 200040, Peoples R China.,xdchen8@hotmail.com
英文摘要	Broussonetia papyrifera (Chinese mulberry) pollen is an important source of allergens in regions surrounding Shanghai, China. To identify and purify major allergens from "B. papyrifera" pollen that reacted with serum antibodies from sensitized patients, "B. papyrifera" pollen was defatted, dried, and extracted proteins were separated using SP cationic exchange or Q anionic exchange columns. Serum samples from 29 allergic patients and 4 healthy controls were collected. Allergens in eluted fractions were identified by Western blot and enzyme-linked immunosorbent assays (ELISA) using serum samples. An inhibitory assay was used to verify allergen-specific antiserum specificity. Serum IgE of 2 patients reacted with a 15 kDa protein band. The protein was eluted with 0.1M NaCl from a SP cationic exchange column. Serum samples from the same patients positively reacted with ELISA plate coated with partially purified 15 kDa protein. Serum IgE of 11 patients reacted with a 72 kDa protein band. The protein was eluted with 0.3M NaCl from a Q anionic exchange column. Serum samples from five patients positively reacted with ELISA coated with partially purified 72 kDa protein. Our preliminary purifications identified two proteins of 72 kDa and 15 kDa as allergens derived from "B. papyrifera" pollen, which reacted with allergic patients' serum IgE.
学科主题	Allergy; Immunology
类目[WOS]	Allergy ; Immunology
关键词[WOS]	DESERT ENVIRONMENT ; MAJOR ALLERGEN
收录类别	SCI
语种	英语
WOS记录号	WOS:000323717200003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/369]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chen, Z,Zhu, N,Chen, XD,et al. Purification and Identification of 72 kDa and 15 kDa Allergens from Broussonetia papyrifera Pollen[J]. IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY,2013,12(4):312-320.
APA	Chen, Z.,Zhu, N.,Chen, XD.,Yang, Y.,Li, YP.,...&Chen, SX.(2013).Purification and Identification of 72 kDa and 15 kDa Allergens from Broussonetia papyrifera Pollen.IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY,12(4),312-320.
MLA	Chen, Z,et al."Purification and Identification of 72 kDa and 15 kDa Allergens from Broussonetia papyrifera Pollen".IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY 12.4(2013):312-320.