Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors

	Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors
	Yu, B; He, ZY; You, P; Han, QW; Xiang, D; Chen, F; Wang, MJ; Liu, CC; Lin, XW; Borjigin, U
刊名	CELL STEM CELL
	2013
卷号	13 期号:3 页码:328-340
通讯作者	Wang, X (reprint author), Inner Mongolia Univ, Natl Educ Minist Mammalian Reprod Biol & Biotechn, Key Lab, Hohhot 010070, Peoples R China.,wangxin_cn@imu.edu.cn ; yphu@smmu.edu.cn
英文摘要	Recent studies have demonstrated direct reprogramming of fibroblasts into a range of somatic cell types, but to date stem or progenitor cells have only been reprogrammed for the blood and neuronal lineages. We previously reported generation of induced hepatocyte-like (iHep) cells by transduction of Gata4, Hnf1 alpha, and Foxa3 in p19 Arf null mouse embryonic fibroblasts (MEFs). Here, we show that Hnf1 beta and Foxa3, liver organogenesis transcription factors, are sufficient to reprogram MEFs into induced hepatic stem cells (iHepSCs). iHepSCs can be stably expanded in vitro and possess the potential of bidirectional differentiation into both hepatocytic and cholangiocytic lineages. In the injured liver of fumarylacetoacetate hydrolase (Fah)-deficient mice, repopulating iHepSCs become hepatocyte-like cells. They also engraft as cholangiocytes into bile ducts of mice with DDC-induced bile ductular injury. Lineage conversion into bipotential expandable iHepSCs provides a strategy to enable efficient derivation of both hepatocytes and cholangiocytes for use in disease modeling and tissue engineering.
学科主题	Cell Biology
类目[WOS]	Cell & Tissue Engineering ; Cell Biology
关键词[WOS]	LIVER PROGENITOR CELLS ; DIRECT CONVERSION ; MOUSE FIBROBLASTS ; TRANSCRIPTION FACTORS ; DOPAMINERGIC-NEURONS ; MICE ; REGENERATION ; REPOPULATION ; HEPATOCYTES ; 3,5-DIETHOXYCARBONYL-1,4-DIHYDROCOLLIDINE
收录类别	SCI
语种	英语
WOS记录号	WOS:000329570800010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/352]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yu, B,He, ZY,You, P,et al. Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors[J]. CELL STEM CELL,2013,13(3):328-340.
APA	Yu, B.,He, ZY.,You, P.,Han, QW.,Xiang, D.,...&Hu, YP.(2013).Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors.CELL STEM CELL,13(3),328-340.
MLA	Yu, B,et al."Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors".CELL STEM CELL 13.3(2013):328-340.