Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11

	Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11
	Gu, YN; Liu, Q; Chen, PY; Guo, CY; Liu, Y; Zhao, YF; Zhang, YL; Lin, DH
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
	2013
卷号	1834 期号:1 页码:42011
关键词	Lipocalin Lcn11 Dimer Disulfide bond Ligand-binding beta-barrel
通讯作者	Lin, DH (reprint author), Xiamen Univ, Key Lab Chem Biol Fujian Prov, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China.,dhlin@xmu.edu.cn
英文摘要	Lipocalin 11 (Lcn11), a recently identified member of the lipocalin family, potentially plays crucial physiological roles in male reproduction. In this present work, we cloned, expressed and purified the rat Lcnl11 (rLcn11) protein in Escherichia coli. A C59A/C156A substitution was introduced to ameliorate the misfolding and aggregation problem associated with the wild-type protein. From circular dichroism and non-reducing SDS-PAGE, we characterized the conformational properties of rLcn11 as a typical lipocalin scaffold with the conserved disulfide bridge. The results obtained from size-exclusion chromatography, cross-linking experiment and dynamic light scattering analysis indicate that the recombinant rLcn11 protein forms dimer in neutral solution. By using fluorescent probe 8-anilino-1-naphtahlene sulfonic acid (ANS), we found rLcn11 might contain multiple hydrophobic binding sites for ligand binding. Similarly to the odorant-binding protein, rLcn11 processes a moderate affinity for binding 1-aminoanthracene (AMA), implying that Lcn11 might work as a dimeric chemoreception protein in male reproductive system. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	EQU C 1 ; BETA-LACTOGLOBULIN ; URINARY PROTEINS ; FLUORESCENCE SPECTROSCOPY ; 3-DIMENSIONAL STRUCTURE ; STRUCTURAL-CHANGES ; PHEROMONE-BINDING ; SITES ; DIMER ; IDENTIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000314620400001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/322]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gu, YN,Liu, Q,Chen, PY,et al. Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2013,1834(1):42011.
APA	Gu, YN.,Liu, Q.,Chen, PY.,Guo, CY.,Liu, Y.,...&Lin, DH.(2013).Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1834(1),42011.
MLA	Gu, YN,et al."Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1834.1(2013):42011.