The PB2 E627K mutation contributes to the high polymerase activity and enhanced replication of H7N9 influenza virus

	The PB2 E627K mutation contributes to the high polymerase activity and enhanced replication of H7N9 influenza virus
	Zhang, H; Li, XY; Guo, J; Li, L; Chang, C; Li, YY; Bian, C; Xu, K; Chen, HL; Sun, B
刊名	JOURNAL OF GENERAL VIROLOGY
	2014
卷号	95 期号:1 页码:779-786
通讯作者	Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Pasteur Shanghai, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China.,hlchen1@yahoo.com ; bsun@sibs.ac.cn
英文摘要	Human infection by H7N9 influenza virus was first identified in China in March 2013. As of 12 August 2013, a total of 135 documented cases with 44 fatalities had been reported. Genetic and laboratory analyses of the novel H7N9 viruses isolated from patients indicate that these viruses possess several polymerase gene mutations previously associated with human adaptation and potential pandemic capabilities. However, the function of these mutations in the emergence and pathogenicity of the viruses is not well known. In this study, we demonstrate that the PB2 E627K mutation, which occurs in over 70% of the H7N9 patient isolates, promotes the replication of H7N9 virus by enhancing PB2 polymerase activity and enhances virulence in mice. Our results show the PB2 E627K mutation has played an important role in this H7N9 influenza outbreak and in the pathogenicity of the H7N9 virus.
学科主题	Biotechnology & Applied Microbiology; Virology
类目[WOS]	Biotechnology & Applied Microbiology ; Virology
关键词[WOS]	HUMAN-CELLS ; A VIRUS ; VIRAL POLYMERASE ; BRITISH-COLUMBIA ; HUMAN INFECTION ; HUMAN ILLNESS ; HOST-RANGE ; TRANSMISSION ; ADAPTATION ; FERRETS
收录类别	SCI
语种	英语
WOS记录号	WOS:000338177700002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/301]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, H,Li, XY,Guo, J,et al. The PB2 E627K mutation contributes to the high polymerase activity and enhanced replication of H7N9 influenza virus[J]. JOURNAL OF GENERAL VIROLOGY,2014,95(1):779-786.
APA	Zhang, H.,Li, XY.,Guo, J.,Li, L.,Chang, C.,...&Sun, B.(2014).The PB2 E627K mutation contributes to the high polymerase activity and enhanced replication of H7N9 influenza virus.JOURNAL OF GENERAL VIROLOGY,95(1),779-786.
MLA	Zhang, H,et al."The PB2 E627K mutation contributes to the high polymerase activity and enhanced replication of H7N9 influenza virus".JOURNAL OF GENERAL VIROLOGY 95.1(2014):779-786.