MicroRNA-mediated repression of nonsense mRNAs | |
Zhao, Y; Lin, JM; Xu, BY; Hu, SD; Zhang, X; Wu, LG | |
刊名 | ELIFE |
2014 | |
卷号 | 3期号:1页码:42006 |
通讯作者 | Wu, LG (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,lgwu@sibcb.ac.cn |
英文摘要 | Numerous studies have established important roles for microRNAs (miRNAs) in regulating gene expression. Here, we report that miRNAs also serve as a surveillance system to repress the expression of nonsense mRNAs that may produce harmful truncated proteins. Upon recognition of the premature termination codon by the translating ribosome, the downstream portion of the coding region of an mRNA is redefined as part of the 3' untranslated region; as a result, the miRNA-responsive elements embedded in this region can be detected by miRNAs, triggering accelerated mRNA deadenylation and translational inhibition. We demonstrate that naturally occurring cancer-causing APC (adenomatous polyposis coli) nonsense mutants which escape nonsense-mediated mRNA decay (NMD) are repressed by miRNA-mediated surveillance. In addition, we show that miRNA-mediated surveillance and exon-exon junction complex-mediated NMD are not mutually exclusive and act additively to enhance the repressive activity. Therefore, we have uncovered a new role for miRNAs in repressing nonsense mutant mRNAs. |
学科主题 | Life Sciences & Biomedicine - Other Topics |
类目[WOS] | Biology |
关键词[WOS] | INTRON RETENTION ; CODING REGIONS ; OVARIAN-CANCER ; APC MUTATIONS ; COLORECTAL TUMORS ; BINDING-PROTEIN ; HUMAN-CELLS ; C-FOS ; DECAY ; GENE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000341250400002 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/296] |
专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
推荐引用方式 GB/T 7714 | Zhao, Y,Lin, JM,Xu, BY,et al. MicroRNA-mediated repression of nonsense mRNAs[J]. ELIFE,2014,3(1):42006. |
APA | Zhao, Y,Lin, JM,Xu, BY,Hu, SD,Zhang, X,&Wu, LG.(2014).MicroRNA-mediated repression of nonsense mRNAs.ELIFE,3(1),42006. |
MLA | Zhao, Y,et al."MicroRNA-mediated repression of nonsense mRNAs".ELIFE 3.1(2014):42006. |
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