The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis

	The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis
	Zhang, M; Zhou, H; Zheng, CX; Xiao, J; Zuo, EW; Liu, WH; Xie, D; Shi, YF; Wu, CL; Wang, HY
刊名	SCIENTIFIC REPORTS
	2014
卷号	4 期号:1 页码:5936-5936
通讯作者	Li, JS (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Grp Epigenet Reprogrammi, State Key Lab Cell Biol,Shanghai Key Lab Mol Andr, Shanghai 200031, Peoples R China.,jsli@sibcb.ac.cn
英文摘要	C-kit positive (c-kit(+)) cells are usual tissue-specific stem cells. However, in postnatal testis, undifferentiated spermatogonial stem cells (SSCs) are c-kit negative (c-kit(-)) and activation of c-kit represents the start of SSC differentiation, leaving an intriguing question whether other c-kit(+) cells exist and participate in the postnatal development of testis. To this end, a feasible system for testicular reconstitution, in which a specific type of cells can be manipulated, is needed. Here, we first establish de novo morphogenesis of testis by subcutaneous injection of testicular cells from neonatal testes into the backs of nude mice. We observe testicular tissue formation and spermatogenesis from all injected sites. Importantly, functional spermatids can be isolated from these testicular tissues. Using this system, we systemically analyze the roles of c-kit(+) cells in testicular reconstitution and identify a small population of cells (c-kit(+): CD140a(+): F4/80(+)), which express typical markers of macrophages, are critical for de novo morphogenesis of testis. Interestingly, we demonstrate that these cells are gradually replaced by peripheral blood cells of recipient mice during the morphogenesis of testis. Thus, we develop a system, which may mimic the complete developmental process of postnatal testis, for investigating the testicular development and spermatogenesis.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	GERMLINE STEM-CELLS ; FACTOR RECEPTOR/C-KIT ; IN-VIVO ; MOUSE SPERMATOGENESIS ; SEMINIFEROUS TUBULES ; LEYDIG-CELLS ; W-LOCUS ; MACROPHAGES ; EXPRESSION ; SPERMATOGONIA
收录类别	SCI
语种	英语
WOS记录号	WOS:000339941700002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/287]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, M,Zhou, H,Zheng, CX,et al. The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis[J]. SCIENTIFIC REPORTS,2014,4(1):5936-5936.
APA	Zhang, M.,Zhou, H.,Zheng, CX.,Xiao, J.,Zuo, EW.,...&Li, JS.(2014).The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis.SCIENTIFIC REPORTS,4(1),5936-5936.
MLA	Zhang, M,et al."The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis".SCIENTIFIC REPORTS 4.1(2014):5936-5936.