Androgenic regulation of beta-defensins in the mouse epididymis

	Androgenic regulation of beta-defensins in the mouse epididymis
	Hu, SG; Zou, M; Yao, GX; Ma, WB; Zhu, QL; Li, XQ; Chen, ZJ; Sun, Y
刊名	REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
	2014
卷号	12 期号:1 页码:76-76
关键词	Androgen Androgen receptor Epididymis Beta-defensins
通讯作者	Sun, Y (reprint author), Shanghai Jiao Tong Univ, Renji Hosp, Ctr Reprod Med, Sch Med,Shanghai Key Lab Assisted Reprod & Reprod, Shanghai 200135, Peoples R China.,syun163@163.com
英文摘要	Background: The majority of beta-defensin family members are exclusively expressed in the epididymis, and some members have been shown to play essential roles in sperm maturation and fertility in rats, mice and humans. Therefore, beta-defensins are hypothesized to be potential targets for contraception and infertility diagnosis and treatment. Clarifying the regulatory mechanisms for the expression of these genes is necessary. Androgen/androgen receptor (AR) signaling plays an important regulatory role in epididymal structure and function. However, very little is known about the androgenic regulation on the production and secretion of the epididymal beta-defensins. Methods: The expression of beta-defensins was detected by quantitative RT-PCR. The androgen dependence of beta-defensins was determined by bilateral orchiectomy and androgen supplementation. The androgen response elements (AREs) in the promoters of beta-defensins were identified using the MatInspector software. The binding of AR to AREs was assayed by ChIP-PCR/qPCR. Results: We demonstrated that 23 mouse caput epididymal beta-defensins were differentially regulated by androgen/androgen receptor. Six genes, Defb18, 19, 20, 39, 41, and 42, showed full regulation by androgens. Ten genes, Defb15, 30, 34, 37, 40, 45, 51, 52, 22 and Spag11a, were partially regulated by androgens. Defb15, 18, 19, 20, 30, 34, 37, 39, 41, 42, 22 and Spag11a were associated with androgen receptor binding sites in their promoter or intronic regions, indicating direct regulation of AR. Six genes, Defb1, 12, 13, 29, 35, and spag11b/c, exhibited an androgen-independent expression pattern. One gene, Defb25, was highly dependent on testicular factors rather on androgens. Conclusions: The present study provides novel insights into the mechanisms of androgen regulation on epididymal beta-defensins, enabling a better understanding of the function of beta-defensins in sperm maturation and fertility.
学科主题	Endocrinology & Metabolism; Reproductive Biology
类目[WOS]	Endocrinology & Metabolism ; Reproductive Biology
关键词[WOS]	MALE REPRODUCTIVE-TRACT ; GENE CLUSTERS ; EXPRESSION ; IDENTIFICATION ; DISCOVERY ; RECEPTOR ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000340062000001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/278]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, SG,Zou, M,Yao, GX,et al. Androgenic regulation of beta-defensins in the mouse epididymis[J]. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY,2014,12(1):76-76.
APA	Hu, SG.,Zou, M.,Yao, GX.,Ma, WB.,Zhu, QL.,...&Sun, Y.(2014).Androgenic regulation of beta-defensins in the mouse epididymis.REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY,12(1),76-76.
MLA	Hu, SG,et al."Androgenic regulation of beta-defensins in the mouse epididymis".REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY 12.1(2014):76-76.