R-spondin1 is a novel hormone mediator for mammary stem cell self-renewal

	R-spondin1 is a novel hormone mediator for mammary stem cell self-renewal
	Cai, CG; Yu, QC; Jiang, WM; Liu, W; Song, WQ; Yu, H; Zhang, L; Yang, Y; Zeng, YA
刊名	GENES & DEVELOPMENT
	2014
卷号	28 期号:20 页码:2205-2218
关键词	mammary stem cell niche luminal cell hormone Rspo1 Wnt4
通讯作者	Zeng, YA (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,yzeng@sibcb.ac.cn
英文摘要	Signals from the niche play pivotal roles in regulating adult stem cell self-renewal. Previous studies indicated that the steroid hormones can expand mammary stem cells (MaSCs) in vivo. However, the facilitating local niche factors that directly contribute to the MaSC expansion remain unclear. Here we identify R-spondin1 (Rspo1) as a novel hormonal mediator in the mammary gland. Pregnancy and hormonal treatment up-regulate Rspo1 expression. Rspo1 cooperates with another hormonal mediator, Wnt4, to promote MaSC self-renewal through Wnt/beta-catenin signaling. Knockdown of Rspo1 and Wnt4 simultaneously abolishes the stem cell reconstitution ability. In culture, hormonal treatment that stimulates the expression of both Rspo1 and Wnt4 can completely substitute for exogenous Wnt proteins, potently expand MaSCs, and maintain their full development potential in transplantation. Our data unveil the intriguing concept that hormones induce a collaborative local niche environment for stem cells.
学科主题	Cell Biology; Developmental Biology; Genetics & Heredity
类目[WOS]	Cell Biology ; Developmental Biology ; Genetics & Heredity
关键词[WOS]	GLAND DEVELOPMENT ; WNT/BETA-CATENIN ; BETA-CATENIN ; IN-VITRO ; GENE-EXPRESSION ; WNT PROTEINS ; RECEPTOR ; MOUSE ; CANCER ; GROWTH
收录类别	SCI
语种	英语
WOS记录号	WOS:000343640000002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/261]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Cai, CG,Yu, QC,Jiang, WM,et al. R-spondin1 is a novel hormone mediator for mammary stem cell self-renewal[J]. GENES & DEVELOPMENT,2014,28(20):2205-2218.
APA	Cai, CG.,Yu, QC.,Jiang, WM.,Liu, W.,Song, WQ.,...&Zeng, YA.(2014).R-spondin1 is a novel hormone mediator for mammary stem cell self-renewal.GENES & DEVELOPMENT,28(20),2205-2218.
MLA	Cai, CG,et al."R-spondin1 is a novel hormone mediator for mammary stem cell self-renewal".GENES & DEVELOPMENT 28.20(2014):2205-2218.