A smartphone controlled handheld microfluidic liquid handling system

	A smartphone controlled handheld microfluidic liquid handling system
	Li, BC; Li, L; Guan, A; Dong, Q; Ruan, KC; Hu, RG; Li, ZY
刊名	LAB ON A CHIP
	2014
卷号	14 期号:20 页码:4085-4092
通讯作者	Li, ZY (reprint author), George Washington Univ, Dept Biomed Engn, Nanophoton & Microfluid Lab, Washington, DC 20052 USA.,zhenyu@gwu.edu
英文摘要	Microfluidics and lab-on-a-chip technologies have made it possible to manipulate small volume liquids with unprecedented resolution, automation and integration. However, most current microfluidic systems still rely on bulky off-chip infrastructures such as compressed pressure sources, syringe pumps and computers to achieve complex liquid manipulation functions. Here, we present a handheld automated microfluidic liquid handling system controlled by a smartphone, which is enabled by combining elastomeric on-chip valves and a compact pneumatic system. As a demonstration, we show that the system can automatically perform all the liquid handling steps of a bead-based HIV1 p24 sandwich immunoassay on a multi-layer PDMS chip without any human intervention. The footprint of the system is 6 x 10.5 x 16.5 cm, and the total weight is 829 g including battery. Powered by a 12.8 V 1500 mAh Li battery, the system consumed 2.2 W on average during the immunoassay and lasted for 8.7 h. This handheld microfluidic liquid handling platform is generally applicable to many biochemical and cell-based assays requiring complex liquid manipulation and sample preparation steps such as FISH, PCR, flow cytometry and nucleic acid sequencing. In particular, the integration of this technology with read-out biosensors may help enable the realization of the long-sought Tricorder-like handheld in vitro diagnostic (IVD) systems.
学科主题	Biochemistry & Molecular Biology; Chemistry; Science & Technology - Other Topics
类目[WOS]	Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
关键词[WOS]	LARGE-SCALE INTEGRATION ; ACTUATION ; DEVICES ; VALVES ; PUMPS
收录类别	SCI
语种	英语
WOS记录号	WOS:000342866400022
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/260]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, BC,Li, L,Guan, A,et al. A smartphone controlled handheld microfluidic liquid handling system[J]. LAB ON A CHIP,2014,14(20):4085-4092.
APA	Li, BC.,Li, L.,Guan, A.,Dong, Q.,Ruan, KC.,...&Li, ZY.(2014).A smartphone controlled handheld microfluidic liquid handling system.LAB ON A CHIP,14(20),4085-4092.
MLA	Li, BC,et al."A smartphone controlled handheld microfluidic liquid handling system".LAB ON A CHIP 14.20(2014):4085-4092.