Lysophosphatidic acid acts as a nutrient-derived developmental cue to regulate early hematopoiesis

	Lysophosphatidic acid acts as a nutrient-derived developmental cue to regulate early hematopoiesis
	Li, HS; Yue, R; Wei, B; Gao, G; Du, JL; Pei, G
刊名	EMBO JOURNAL
	2014
卷号	33 期号:12 页码:1383-1396
关键词	embryonic stem cell hemangioblast hematopoiesis LPA zebrafish
通讯作者	Pei, G (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, State Key Lab Cell Biol,Inst Biochem & Cell Biol, Shanghai, Peoples R China.,gpei@sibs.ac.cn
英文摘要	Primitive hematopoiesis occurs in the yolk sac blood islands during vertebrate embryogenesis, where abundant phosphatidylcholines (PC) are available as important nutrients for the developing embryo. However, whether these phospholipids also generate developmental cues to promote hematopoiesis is largely unknown. Here, we show that lysophosphatidic acid (LPA), a signaling molecule derived from PC, regulated hemangioblast formation and primitive hematopoiesis. Pharmacological and genetic blockage of LPA receptor 1 (LPAR1) or autotoxin (ATX), a secretory lysophospholipase that catalyzes LPA production, inhibited hematopoietic differentiation of mouse embryonic stem cells and impaired the formation of hemangioblasts. Mechanistic experiments revealed that the regulatory effect of ATX-LPA signaling was mediated by PI3K/Akt-Smad pathway. Furthermore, during in vivo embryogenesis in zebrafish, LPA functioned as a developmental cue for hemangioblast formation and primitive hematopoiesis. Taken together, we identified LPA as an important nutrient-derived developmental cue for primitive hematopoiesis as well as a novel mechanism of hemangioblast regulation.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	EMBRYONIC STEM-CELLS ; BONE MORPHOGENETIC PROTEIN-4 ; ENDOTHELIAL-CELLS ; LYSOPHOSPHOLIPASE-D ; HEMANGIOBLAST DEVELOPMENT ; HAEMOGENIC ENDOTHELIUM ; VASCULAR MATURATION ; COUPLED RECEPTOR ; COMMON PRECURSOR ; PRIMITIVE STREAK
收录类别	SCI
语种	英语
WOS记录号	WOS:000338024100011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/253]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, HS,Yue, R,Wei, B,et al. Lysophosphatidic acid acts as a nutrient-derived developmental cue to regulate early hematopoiesis[J]. EMBO JOURNAL,2014,33(12):1383-1396.
APA	Li, HS,Yue, R,Wei, B,Gao, G,Du, JL,&Pei, G.(2014).Lysophosphatidic acid acts as a nutrient-derived developmental cue to regulate early hematopoiesis.EMBO JOURNAL,33(12),1383-1396.
MLA	Li, HS,et al."Lysophosphatidic acid acts as a nutrient-derived developmental cue to regulate early hematopoiesis".EMBO JOURNAL 33.12(2014):1383-1396.