NEDD9 promotes lung cancer metastasis through epithelial- mesenchymal transition

	NEDD9 promotes lung cancer metastasis through epithelial- mesenchymal transition
	Jin, YJ; Li, F; Zheng, C; Wang, Y; Fang, ZY; Guo, CC; Wang, XJ; Liu, HY; Deng, L; Li, C
刊名	INTERNATIONAL JOURNAL OF CANCER
	2014
卷号	134 期号:10 页码:2294-2304
关键词	NEDD9 epithelial-mesenchymal transition lung cancer metastasis
通讯作者	Ji, HB (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,fengyan01@sibs.ac.cn ; hbji@sibcb.ac.cn
英文摘要	Metastasis is the major cause for high mortality of lung cancer with the underlying mechanisms poorly understood. The scaffolding protein neural precursor cell expressed, developmentally down-regulated 9 (NEDD9) has been identified as a pro-metastasis gene in several types of cancers including melanoma and breast cancer. However, the exact role and related mechanism of NEDD9 in regulating lung cancer metastasis still remain largely unknown. Here, we demonstrate that NEDD9 knockdown significantly inhibits migration, invasion and metastasis of lung cancer cells in vitro and in vivo. The pro-metastasis role of Nedd9 in lung cancer is further supported by studies in mice models of spontaneous cancer metastasis. Moreover, we find that NEDD9 promotes lung cancer cell migration and invasion through the induction of epithelial-mesenchymal transition (EMT) potentially via focal adhesion kinase activation. More importantly, NEDD9 expression inversely correlates with E-cadherin expression in human lung cancer specimens, consistent with the findings from in vitro studies. Taken together, this study highlights that NEDD9 is an important mediator promotes lung cancer metastasis via EMT. What's new? The scaffolding protein NEDD9 regulates signaling molecules involved in cell invasion and metastasis, activities that are promoted in melanoma and breast cancer by upregulation of the NEDD9 gene. The present study examined the role of NEDD9 in lung cancer metastasis. In vitro and in vivo experiments demonstrated that knockdown of NEDD9 inhibits lung cancer cell migration, invasion, and metastasis, while mechanistic exploration revealed that NEDD9 promotes those activities via induction of epithelial-mesenchymal transition (EMT). Expression of NEDD9 was found to be inversely correlated with E-cadherin expression in human lung cancer specimens.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	DOCKING PROTEIN HEF1 ; BETA-CATENIN ; CELL-CYCLE ; E-CADHERIN ; EXPRESSION ; PROGRESSION ; LKB1 ; MUTATIONS ; MIGRATION ; NETWORKS
收录类别	SCI
语种	英语
WOS记录号	WOS:000332309700004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/237]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Jin, YJ,Li, F,Zheng, C,et al. NEDD9 promotes lung cancer metastasis through epithelial- mesenchymal transition[J]. INTERNATIONAL JOURNAL OF CANCER,2014,134(10):2294-2304.
APA	Jin, YJ.,Li, F.,Zheng, C.,Wang, Y.,Fang, ZY.,...&Ji, HB.(2014).NEDD9 promotes lung cancer metastasis through epithelial- mesenchymal transition.INTERNATIONAL JOURNAL OF CANCER,134(10),2294-2304.
MLA	Jin, YJ,et al."NEDD9 promotes lung cancer metastasis through epithelial- mesenchymal transition".INTERNATIONAL JOURNAL OF CANCER 134.10(2014):2294-2304.