Crystal Structure of the Rab9A-RUTBC2 RBD Complex Reveals the Molecular Basis for the Binding Specificity of Rab9A with RUTBC2

	Crystal Structure of the Rab9A-RUTBC2 RBD Complex Reveals the Molecular Basis for the Binding Specificity of Rab9A with RUTBC2
	Zhang, Z; Wang, SS; Shen, T; Chen, JY; Ding, JP
刊名	STRUCTURE
	2014
卷号	22 期号:10 页码:1408-1420
通讯作者	Ding, JP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jpding@sibcb.ac.cn
英文摘要	Rab9 plays a vital role in regulating the transport of mannose 6-phosphate receptors from late endosomes to the trans-Golgi network through interactions with various effectors. Here, we report the crystal structure of GTP-bound Rab9A in complex with the Rab-binding domain (RBD) of the effector RUTBC2. RUTBC2 RBD assumes a pleckstrin homology domain fold that uses a binding site consisting of mainly b1 and the h1 insertion to interact with the switch and interswitch regions of Rab9A. The C-terminal hypervariable region of Rab9A is disordered and thus not required for RUTBC2 binding. The conformational plasticity of the switch and interswitch regions of Rab9A primarily determines the specificity for RUTBC2. Our biochemical and biological data confirm these findings and further show that Rab9B can bind to RUTBC2 probably in a similar manner as Rab9A. These results together reveal the molecular basis for the binding specificity of Rab9A with RUTBC2.
学科主题	Biochemistry & Molecular Biology; Biophysics; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	PLECKSTRIN HOMOLOGY DOMAINS ; TRANS-GOLGI NETWORK ; MEMBRANE RECRUITMENT ; GTPASE RECOGNITION ; PH DOMAIN ; EFFECTOR ; FAMILY ; PROTEIN ; ENDOSOMES ; PHOSPHOINOSITIDES
收录类别	SCI
语种	英语
WOS记录号	WOS:000343586000006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/230]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, Z,Wang, SS,Shen, T,et al. Crystal Structure of the Rab9A-RUTBC2 RBD Complex Reveals the Molecular Basis for the Binding Specificity of Rab9A with RUTBC2[J]. STRUCTURE,2014,22(10):1408-1420.
APA	Zhang, Z,Wang, SS,Shen, T,Chen, JY,&Ding, JP.(2014).Crystal Structure of the Rab9A-RUTBC2 RBD Complex Reveals the Molecular Basis for the Binding Specificity of Rab9A with RUTBC2.STRUCTURE,22(10),1408-1420.
MLA	Zhang, Z,et al."Crystal Structure of the Rab9A-RUTBC2 RBD Complex Reveals the Molecular Basis for the Binding Specificity of Rab9A with RUTBC2".STRUCTURE 22.10(2014):1408-1420.