Bantam is essential for Drosophila intestinal stem cell proliferation in response to Hippo signaling

	Bantam is essential for Drosophila intestinal stem cell proliferation in response to Hippo signaling
	Huang, HL; Li, JH; Hu, LX; Ge, L; Ji, HB; Zhao, Y; Zhang, L
刊名	DEVELOPMENTAL BIOLOGY
	2014
卷号	385 期号:2 页码:211-219
关键词	Bantam MicroRNA Drosophila Intestinal stem cell Hippo pathway
通讯作者	Zhao, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Yue Yang Rd, Shanghai 200031, Peoples R China.,yunzhao@sibcb.ac.cn ; rayzhang@sibcb.ac.cn
英文摘要	The Drosophila midgut has emerged as an attractive model system to study stem cell biology. Extensive studies have been carried out to investigate the mechanisms of how the signaling pathways integrate to regulate intestinal stem cells (ISCs), yet, whether the microRNAs are involved in ISC self-renewal and maintenance is unknown. Here we demonstrate that the bantam microRNA is expressed specifically at high levels in Drosophila midgut precursor cells (including ISCs and enteroblasts) and secretory enteroendocrine cells while at extremely low levels in enterocytes. Furthermore, overexpression of bantam microRNA results in increase of the division of the midgut precursor cells, whereas loss of bantam microRNA decreases their proliferation. The mechanical studies show that bantam microRNA is essential for the Hpo pathway induced cell-autonomous ISC self-renewal, while it is disposable for EGFR and Notch pathways mediated ISC proliferation. More interestingly, we find that bantam microRNA is not required for the Hpo pathway mediated non-cell-autonomous ISC proliferation, revealing a novel mechanism by which the Hpo signaling pathway specifies its transcriptional targets in specific tissue to exhibit its biological functions. (C) 2013 Elsevier Inc. All rights reserved.
学科主题	Developmental Biology
类目[WOS]	Developmental Biology
关键词[WOS]	TEAD/TEF FAMILY ; PATHWAY ; MIDGUT ; REGENERATION ; NOTCH ; REPRESSION ; APOPTOSIS ; MICRORNA ; MIRNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000330205700007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/152]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Huang, HL,Li, JH,Hu, LX,et al. Bantam is essential for Drosophila intestinal stem cell proliferation in response to Hippo signaling[J]. DEVELOPMENTAL BIOLOGY,2014,385(2):211-219.
APA	Huang, HL.,Li, JH.,Hu, LX.,Ge, L.,Ji, HB.,...&Zhang, L.(2014).Bantam is essential for Drosophila intestinal stem cell proliferation in response to Hippo signaling.DEVELOPMENTAL BIOLOGY,385(2),211-219.
MLA	Huang, HL,et al."Bantam is essential for Drosophila intestinal stem cell proliferation in response to Hippo signaling".DEVELOPMENTAL BIOLOGY 385.2(2014):211-219.