Soluble fusion expression, characterization and localization of human beta-defensin 6

	Soluble fusion expression, characterization and localization of human beta-defensin 6
	Xin, AJ; Zhao, Y; Yu, HG; Shi, HJ; Liu, HY; Diao, H; Zhang, YL
刊名	MOLECULAR MEDICINE REPORTS
	2014
卷号	9 期号:1 页码:149-155
关键词	human beta-defensin 6 antimicrobial peptide intein purification binding with heparin and lipopolysaccharide
通讯作者	Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,diaohua@gmail.com ; ylzhang@sibs.ac.cn
英文摘要	Human -defensin 6 (DEFB106) is an antimicrobial peptide expressed in the epididymis, testis and lung, which indicates that DEFB106 may be involved in innate immunity and fertility. However, as a -defensin, this protein has not been well characterized. Using an intein-mediated fusion expression system, the recombinant DEFB106 was expressed and purified (yield, 3-5 mg/l) under optimized conditions. The purified protein was characterized using mass spectrometry and circular dichroism spectroscopy. The measured molecular weight was consistent with its theoretical value and the predominant secondary structure was -sheet, the common structure of -defensin family members. The purified DEFB106 showed antimicrobial activity against not only Escherichia coli (E. coli) and Candida albicans (C. albicans) SC5314, but also Staphylococcus aureus (S. aureus) CMCC26003. Furthermore, it exhibited a high affinity for heparin and lipopolysaccharide. In addition, it was determined that native DEFB106 was located in the epididymis, bone marrow and skin. These observations may aid in the determination of the physiological and pathological functions of DEFB106.
学科主题	Oncology; Research & Experimental Medicine
类目[WOS]	Oncology ; Medicine, Research & Experimental
关键词[WOS]	ESCHERICHIA-COLI ; 2 HBD2 ; PEPTIDES ; BINDING ; IDENTIFICATION ; EVOLUTION ; MEMBRANES ; INSERTION ; LIGATION ; PROTEINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000330783700024
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/129]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xin, AJ,Zhao, Y,Yu, HG,et al. Soluble fusion expression, characterization and localization of human beta-defensin 6[J]. MOLECULAR MEDICINE REPORTS,2014,9(1):149-155.
APA	Xin, AJ.,Zhao, Y.,Yu, HG.,Shi, HJ.,Liu, HY.,...&Zhang, YL.(2014).Soluble fusion expression, characterization and localization of human beta-defensin 6.MOLECULAR MEDICINE REPORTS,9(1),149-155.
MLA	Xin, AJ,et al."Soluble fusion expression, characterization and localization of human beta-defensin 6".MOLECULAR MEDICINE REPORTS 9.1(2014):149-155.