The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa

	The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa
	Yang, NN; Ding, ST; Chen, FF; Zhang, X; Xia, YJ; Di, HX; Cao, Q; Deng, X; Wu, M; Wong, CCL
刊名	MOLECULAR MICROBIOLOGY
	2015
卷号	96 期号:3 页码:526-547
通讯作者	Lan, LF (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd,Pudong Zhangjiang Hitech Pk, Shanghai 201203, Peoples R China.,llan@simm.ac.cn
英文摘要	Rhamnolipid acts as a virulence factor during Pseudomonas aeruginosa infection. Here, we show that deletion of the catabolite repression control (crc) gene in P.aeruginosa leads to a rhamnolipid-negative phenotype. This effect is mediated by the down-regulation of rhl quorum sensing (QS). We discover that a disruption of the gene encoding the Lon protease entirely offsets the effect of crc deletion on the production of both rhamnolipid and rhlQS signal C4-HSL. Crc is unable to bind lon mRNAin vitro in the absence of the RNA chaperon Hfq, while Crc contributes to Hfq-mediated repression of the lon gene expression at a posttranscriptional level. Deletion of crc, which results in up-regulation of lon, significantly reduces the in vivo stability and abundance of the RhlI protein that synthesizes C4-HSL, causing the attenuation of rhlQS. Lon is also capable of degrading the RhlI protein in vitro. In addition, constitutive expression of rhlI suppresses the defects of the crc deletion mutant in rhamnolipid, C4-HSL and virulence on lettuce leaves. This study therefore uncovers a novel posttranscriptional regulatory cascade, Crc-Hfq/Lon/RhlI, for the regulation of rhamnolipid production and rhlQS in P.aeruginosa.
学科主题	Biochemistry & Molecular Biology; Microbiology
类目[WOS]	Biochemistry & Molecular Biology ; Microbiology
关键词[WOS]	CARBON CATABOLITE REPRESSION ; ESCHERICHIA-COLI ; GLOBAL REGULATOR ; BIOSURFACTANT SYNTHESIS ; STAPHYLOCOCCUS-AUREUS ; BACTERIAL VIRULENCE ; BIOFILM FORMATION ; QUALITY-CONTROL ; RNA-SEQ ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000353895800007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/31]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, NN,Ding, ST,Chen, FF,et al. The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa[J]. MOLECULAR MICROBIOLOGY,2015,96(3):526-547.
APA	Yang, NN.,Ding, ST.,Chen, FF.,Zhang, X.,Xia, YJ.,...&Lan, LF.(2015).The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa.MOLECULAR MICROBIOLOGY,96(3),526-547.
MLA	Yang, NN,et al."The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa".MOLECULAR MICROBIOLOGY 96.3(2015):526-547.