MIWI and piRNA-mediated cleavage of messenger RNAs in mouse testes

	MIWI and piRNA-mediated cleavage of messenger RNAs in mouse testes
	Zhang, P; Kang, JY; Gou, LT; Wang, JJ; Xue, YC; Skogerboe, G; Dai, P; Huang, DW; Chen, RS; Fu, XD
刊名	CELL RESEARCH
	2015
卷号	25 期号:2 页码:193-207
关键词	piRNA MIWI sperm
通讯作者	He, SM (reprint author), Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China.,xdfu@ucsd.edu ; mfliu@sibcb.ac.cn ; heshunmin@gmail.com
英文摘要	The piRNA machinery is known for its role in mediating epigenetic silencing of transposons. Recent studies suggest that this function also involves piRNA-guided cleavage of transposon-derived transcripts. As many piRNAs also appear to have the capacity to target diverse mRNAs, this raises the intriguing possibility that piRNAs may act extensively as siRNAs to degrade specific mRNAs. To directly test this hypothesis, we compared mouse PIWI (MIWI)-associated piRNAs with experimentally identified cleaved mRNA fragments from mouse testes, and observed cleavage sites that predominantly occur at position 10 from the 5' end of putative targeting piRNAs. We also noted strong biases for U and A residues at nucleotide positions 1 and 10, respectively, in both piRNAs and mRNA fragments, features that resemble the pattern of piRNA amplification by the 'ping-pong' cycle. Through mapping of MIWI-RNA interactions by CLIP-seq and gene expression profiling, we found that many potential piRNA-targeted mRNAs directly interact with MIWI and show elevated expression levels in the testes of Miwi catalytic mutant mice. Reporter-based assays further revealed the importance of base pairing between piRNAs and mRNA targets and the requirement for both the slicer activity and piRNA-loading ability of MIWI in piRNA-mediated target repression. Importantly, we demonstrated that proper turnover of certain key piRNA targets is essential for sperm formation. Together, these findings reveal the siRNA-like function of the piRNA machinery in mouse testes and its central requirement for male germ cell development and maturation.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	NOVO DNA METHYLATION ; PIWI PROTEINS ; ADULT SPERMATOGENESIS ; DROSOPHILA ; PATHWAY ; BIOGENESIS ; MECHANISM ; GERMLINE ; ELEMENTS ; GENOME
收录类别	SCI
语种	英语
WOS记录号	WOS:000349334100008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/23]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, P,Kang, JY,Gou, LT,et al. MIWI and piRNA-mediated cleavage of messenger RNAs in mouse testes[J]. CELL RESEARCH,2015,25(2):193-207.
APA	Zhang, P.,Kang, JY.,Gou, LT.,Wang, JJ.,Xue, YC.,...&He, SM.(2015).MIWI and piRNA-mediated cleavage of messenger RNAs in mouse testes.CELL RESEARCH,25(2),193-207.
MLA	Zhang, P,et al."MIWI and piRNA-mediated cleavage of messenger RNAs in mouse testes".CELL RESEARCH 25.2(2015):193-207.