Characterization of Two Pollen Allergens of the London Plane Tree in Shanghai

	Characterization of Two Pollen Allergens of the London Plane Tree in Shanghai
	Chen, Z; Yang, Y; Chen, XD; Wu, ZL; Li, SF
刊名	IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY
	2015
卷号	14 期号:2 页码:139-148
关键词	London plane tree Mass spectrometry Pla a 1 Pla a 2 Platanus acerifolia Pollen allergen
通讯作者	Chen, XD (reprint author), Fudan Univ, Shanghai Huashan Hosp, 12 Urumqi Rd M, Shanghai 200433, Peoples R China.,xdchen8@hotmail.com
英文摘要	Platanus acerifolia, London plane tree, a significant source of airborne allergens, is widely grown in Shanghai and other cities in China. In recent decades, little has been known regarding the influence of the allergens on sensitizing the population in the Shanghai area. The aims of this study were to purify and characterize the two major allergens and to confirm the immunological activities of these pollen allergens in Shanghai. Crude extract was purified with a HiTrap SP column and a Sephedex G75 column. Immunodetection was performed with ELISA and immunoblotting. Following gel proteolytic digestion and mass spectrometry, the tandem MS (MS/MS) peptide mass fingerprint was obtained and the MASCOT search engine was used to identify the peptide. The accession number of the interesting homologous data and all the sequence information was acquired by an internet database and the evolutionary trees were drawn with Mega 4.0 software. Two proteins with molecular weights of 43 kDa and 18 kDa were purified from P. acerifolia pollen extract. The purified proteins were identified as pollen allergen Pla a 1 and Pla a 2 via mass spectrometry. The proteins have immunological activities with human IgE antibodies. According to the ELISA results, 12% (5/41) of the subjects were sensitive to Pla a 1 and 9% (4/41) were sensitive to Pla a 2. Pla a 1 and Pla a 2 are thus important allergens for patients with an allergic reaction to P. acerifolia pollen in Shanghai.
学科主题	Allergy; Immunology
类目[WOS]	Allergy ; Immunology
关键词[WOS]	PLATANUS-ACERIFOLIA POLLEN ; LIPID-TRANSFER PROTEIN ; MAJOR ALLERGEN ; IDENTIFICATION ; POLYGALACTURONASE ; PURIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000353710000004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/17]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chen, Z,Yang, Y,Chen, XD,et al. Characterization of Two Pollen Allergens of the London Plane Tree in Shanghai[J]. IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY,2015,14(2):139-148.
APA	Chen, Z,Yang, Y,Chen, XD,Wu, ZL,&Li, SF.(2015).Characterization of Two Pollen Allergens of the London Plane Tree in Shanghai.IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY,14(2),139-148.
MLA	Chen, Z,et al."Characterization of Two Pollen Allergens of the London Plane Tree in Shanghai".IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY 14.2(2015):139-148.