The pH-dependence of photochemical intermediates of O and P in bacteriorhodopsin by continuous light

CORC > 西安光学精密机械研究所 > 中国科学院西安光学精密机械研究所 > 瞬态光学技术国家重点实验室

	The pH-dependence of photochemical intermediates of O and P in bacteriorhodopsin by continuous light
	Wang, LP ; Shen, ZS ; Wang, JW ; Li, BF ; Chen, F ; Yang, WZ ; Feng, XQ
刊名	biochemical and biophysical research communications
	2006-05-12
卷号	343 期号:3 页码:899-903
关键词	bacteriorhodopsin photoequilibrium state pH-dependence O intermediate photocycle laser
英文摘要	the ph-dependence of the o and p intermediates in the photocycle of bacteriorhodopsin (br) on the intensity and duration of the exciting flash was investigated for br glycerol suspensions and br gelatin films. green and red laser flashes (532 and 670 nm) were utilized to generate a photoequilibrium state of br and o at ambient temperature, and uv-vis spectroscopy was used to determine the photoconversion for the br suspensions and films. the maximal concentration of the o intermediate was observed to be ph-dependent and the dependency was most pronounced at a slightly alkaline ph values. the photochemical conversion from the o to p intermediate was investieated for both br suspensions and films. the p intermediate was only found in br gelatin film. these results indicate that br gelatin film may be an attractive candidate for the information storage based on p intermediate. it is possible, with red light, to create photoproducts which are thermally stable at ambient temperature and that can be photochemically erased. (c) 2006 elsevier inc. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; biophysics
研究领域[WOS]	biochemistry & molecular biology ; biophysics
关键词[WOS]	protein residues ; photocycle ; release ; protonation ; asp-85 ; o-630 ; model ; pump
收录类别	SCI
语种	英语
WOS记录号	WOS:000236976800031
公开日期	2015-08-25
内容类型	期刊论文
源URL	[http://ir.opt.ac.cn/handle/181661/25182]
专题	西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位	1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Xian Inst Opt & Fine Mech, Xian 710068, Peoples R China
推荐引用方式 GB/T 7714	Wang, LP,Shen, ZS,Wang, JW,et al. The pH-dependence of photochemical intermediates of O and P in bacteriorhodopsin by continuous light[J]. biochemical and biophysical research communications,2006,343(3):899-903.
APA	Wang, LP.,Shen, ZS.,Wang, JW.,Li, BF.,Chen, F.,...&Feng, XQ.(2006).The pH-dependence of photochemical intermediates of O and P in bacteriorhodopsin by continuous light.biochemical and biophysical research communications,343(3),899-903.
MLA	Wang, LP,et al."The pH-dependence of photochemical intermediates of O and P in bacteriorhodopsin by continuous light".biochemical and biophysical research communications 343.3(2006):899-903.