Identification and Quantification of the Traditional Chinese Medicinal Plant Gentiana macrophylla using Taqman Real-Time PCR

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Identification and Quantification of the Traditional Chinese Medicinal Plant Gentiana macrophylla using Taqman Real-Time PCR
	Xue, Chun-Ying2 ; Li, De-Zhu2 ; Wang, Qing-Zhong 1
刊名	PLANTA MEDICA
	2008-12-01
卷号	74 期号:15 页码:1842-1845
关键词	DNA Gentiana macrophylla Gentianaceae real-time PCR species identification
英文摘要	Gentiana macrophylla Pall. is a commonly used antirheumatic herb. There are four species of Gentiona recorded as herbal drugs in the Chinese Pharmacopoeia. The other species are often marketed as G. macrophylla, and thus the therapeutic effects of G. macrophylla are not achieved. A novel one-step methodology based on real-time polymerase chain reaction (PCR) technology has been developed for the identification of G. macrophylla. This relative quantification methodology does not require a known amount of standard, allowing the analysis of many more samples together. The utilization of real-time PCR does not require sample handling, preventing contamination and resulting in much faster and higher throughput results.
类目[WOS]	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]	Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]	RIBOSOMAL DNA ; AUTHENTICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000262107800017
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/23867]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Yunnan Univ, Sch Chem Sci & Technol, Kunming 610091, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Lab Plant Biodivers & Biogeog, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Xue, Chun-Ying,Li, De-Zhu,Wang, Qing-Zhong. Identification and Quantification of the Traditional Chinese Medicinal Plant Gentiana macrophylla using Taqman Real-Time PCR[J]. PLANTA MEDICA,2008,74(15):1842-1845.
APA	Xue, Chun-Ying,Li, De-Zhu,&Wang, Qing-Zhong.(2008).Identification and Quantification of the Traditional Chinese Medicinal Plant Gentiana macrophylla using Taqman Real-Time PCR.PLANTA MEDICA,74(15),1842-1845.
MLA	Xue, Chun-Ying,et al."Identification and Quantification of the Traditional Chinese Medicinal Plant Gentiana macrophylla using Taqman Real-Time PCR".PLANTA MEDICA 74.15(2008):1842-1845.