Antibiotic Resistance Characteristics of Environmental Bacteria from an Oxytetracycline Production Wastewater Treatment Plant and the Receiving River

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	Antibiotic Resistance Characteristics of Environmental Bacteria from an Oxytetracycline Production Wastewater Treatment Plant and the Receiving River
	Li, Dong; Yu, Tao; Zhang, Yu; Yang, Min; Li, Zhen; Liu, Miaomiao; Qi, Rong
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2010-06
卷号	76 期号:11 页码:3444-3451
中文摘要	We characterized the bacterial populations in surface water receiving effluent from an oxytetracycline (OTC) production plant. Additional sampling sites included the receiving river water 5 km upstream and 20 km downstream from the discharge point. High levels of OTC were found in the wastewater (WW), and the antibiotic was still detectable in river water downstream (RWD), with undetectable levels in river water upstream (RWU). A total of 341 bacterial strains were isolated using nonselective media, with the majority being identified as Gammaproteobacteria. The MICs were determined for 10 antibiotics representing seven different classes of antibiotics, and the corresponding values were significantly higher for the WW and RWD isolates than for the RWU isolates. Almost all bacteria (97%) from the WW and RWD samples demonstrated multidrug-resistant (MDR) phenotypes, while in RWU samples, these were less frequent (28%). The WW and RWD isolates were analyzed for the presence of 23 tetracycline (tet) resistance genes. The majority of isolates (94.2% and 95.4% in WW and RWD, respectively) harbored the corresponding genes, with tet(A) being the most common (67.0%), followed by tet(W), tet(C), tet(J), tet(L), tet(D), tet(Y), and tet(K) (in the range between 21.0% and 40.6%). Class I integrons were detected in the majority of WW and RWD isolates (97.4% and 86.2%, respectively) but were not associated with the tet genes. We hypothesize that the strong selective pressure imposed by a high concentration of OTC contributes to the wide dissemination of tetracycline resistance genes and other antibiotic resistance genes, possibly through mobile genetic elements.
WOS记录号	WOS:000277871400005
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/21202]
专题	生态环境研究中心_环境水质学国家重点实验室
推荐引用方式 GB/T 7714	Li, Dong,Yu, Tao,Zhang, Yu,et al. Antibiotic Resistance Characteristics of Environmental Bacteria from an Oxytetracycline Production Wastewater Treatment Plant and the Receiving River[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2010,76(11):3444-3451.
APA	Li, Dong.,Yu, Tao.,Zhang, Yu.,Yang, Min.,Li, Zhen.,...&Qi, Rong.(2010).Antibiotic Resistance Characteristics of Environmental Bacteria from an Oxytetracycline Production Wastewater Treatment Plant and the Receiving River.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,76(11),3444-3451.
MLA	Li, Dong,et al."Antibiotic Resistance Characteristics of Environmental Bacteria from an Oxytetracycline Production Wastewater Treatment Plant and the Receiving River".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 76.11(2010):3444-3451.