NMR-based metabonomic analysis of the hepatotoxicity induced by combined exposure to PCBs and TCDD in rats

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	NMR-based metabonomic analysis of the hepatotoxicity induced by combined exposure to PCBs and TCDD in rats
	Lu, Chunfeng ; Wang, Yimei ; Sheng, Zhiguo ; Liu, Gang ; Fu, Ze ; Zhao, Jing ; Zhao, Jun ; Yan, Xianzhong ; Zhu, Benzhan ; Peng, Shuangqing
刊名	TOXICOLOGY AND APPLIED PHARMACOLOGY
	2010-11-01
卷号	248 期号:3 页码:178-184
关键词	Polychlorinated biphenyls 2 Combined exposure Hepatotoxicity NMR Metabonomics 3 7 8-Tetrachlorodibenzo-p-dioxin
中文摘要	A metabonomic approach using (1)H NMR spectroscopy was adopted to investigate the metabonomic pattern of rat urine after oral administration of environmental endocrine disruptors (EDs) polychlorinated biphenyls (PCBs) and 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) alone or in combination and to explore the possible hepatotoxic mechanisms of combined exposure to PCBs and TCDD. (1)H NMR spectra of urines collected 24 h before and after exposure were analyzed via pattern recognition by using principal component analysis (PCA). Serum biochemistry and liver histopathology indicated significant hepatotoxicity in the rats of the combined group. The PCA scores plots of urinary (1)H NMR data showed that all the treatment groups could be easily distinguished from the control group, so could the PCBs or TCDD group and the combined group. The loadings plots of the PCA revealed remarkable increases in the levels of lactate, glucose, taurine, creatine. and 2-hydroxy-isovaleric acid and reductions in the levels of 2-oxoglutarate, citrate, succinate, hippurate, and trimethylamine-N-oxide in rat urine after exposure. These changes were more striking in the combined group. The changed metabolites may be considered possible biomarker for the hepatotoxicity. The present study demonstrates that combined exposure to PCBs and TCDD induced significant hepatotoxicity in rats, and mitochondrial dysfunction and fatty acid metabolism perturbations might contribute to the hepatotoxicity. There was good conformity between changes in the urine metabonomic pattern and those in serum biochemistry and liver histopathology. These results showed that the NMR-based metabonomic approach may provide a promising technique for the evaluation of the combined toxicity of EDs. (C) 2010 Elsevier Inc. All rights reserved.
WOS记录号	WOS:000283203600002
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/21113]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Lu, Chunfeng,Wang, Yimei,Sheng, Zhiguo,et al. NMR-based metabonomic analysis of the hepatotoxicity induced by combined exposure to PCBs and TCDD in rats[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2010,248(3):178-184.
APA	Lu, Chunfeng.,Wang, Yimei.,Sheng, Zhiguo.,Liu, Gang.,Fu, Ze.,...&Peng, Shuangqing.(2010).NMR-based metabonomic analysis of the hepatotoxicity induced by combined exposure to PCBs and TCDD in rats.TOXICOLOGY AND APPLIED PHARMACOLOGY,248(3),178-184.
MLA	Lu, Chunfeng,et al."NMR-based metabonomic analysis of the hepatotoxicity induced by combined exposure to PCBs and TCDD in rats".TOXICOLOGY AND APPLIED PHARMACOLOGY 248.3(2010):178-184.