Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds
	Zhao Bin; Baston, David S.; Khan, Elaine; Sorrentino, Claudio; Denison, Michael S.
刊名	SCIENCE CHINA-CHEMISTRY
	2010-05
卷号	53 期号:5 页码:1010-1016
关键词	dioxin reporter gene bioassay CALUX CAFLUX
中文摘要	Reporter genes produce a protein product in transfected cells that can be easily measured in intact or lysed cells and they have been extensively used in numerous basic and applied research applications. Over the past 10 years, reporter gene assays have been widely accepted and used for analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related dioxin-like compounds in various types of matrices, such as biological, environmental, food and feed samples, given that high-resolution instrumental analysis techniques are impractical for large-scale screening analysis. The most sensitive cell-based reporter gene bioassay systems developed are the mechanism-based CALUX (Chemically Activated Luciferase Expression) and CAFLUX (Chemically Activated Fluorescent Expression) bioassays, which utilize recombinant cell lines containing stably transfected dioxin (AhR)-responsive firefly luciferase or enhanced green fluorescent protein (EGFP) reporter genes, respectively. While the current CALUX and CAFLUX bioassays are very sensitive, increasing their lower limit of sensitivity, magnitude of response and dynamic range for chemical detection would significantly increase their utility, particularly for those samples that contain low levels of dioxin-like HAHs (i.e., serum). In this study, we report that the addition of modulators of cell signaling pathways or modification of cell culture conditions results in significant improvement in the magnitude and overall responsiveness of the existing CALUX and CAFLUX cell bioassays.
WOS记录号	WOS:000278263500007
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/21076]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhao Bin,Baston, David S.,Khan, Elaine,et al. Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds[J]. SCIENCE CHINA-CHEMISTRY,2010,53(5):1010-1016.
APA	Zhao Bin,Baston, David S.,Khan, Elaine,Sorrentino, Claudio,&Denison, Michael S..(2010).Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds.SCIENCE CHINA-CHEMISTRY,53(5),1010-1016.
MLA	Zhao Bin,et al."Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds".SCIENCE CHINA-CHEMISTRY 53.5(2010):1010-1016.