Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure
	Ji, Chenglong; Wang, Qing
刊名	JOURNAL OF PROTEOMICS
	2015
卷号	112 页码:83-94
关键词	Ocean acidification Proteomics Metabolomics Oyster Crassostrea gigas
ISSN号	1874-3919
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, YICCAS, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,Shandong Prov, Yantai 264003, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn
产权排序	[Wei, Lei; Wang, Qing; Wu, Huifeng; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, YICCAS, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,Shandong Prov, Yantai 264003, Peoples R China; [Wei, Lei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
中文摘要	The gradually increased atmospheric CO2 partial pressure (pCO(2)) has thrown the carbonate chemistry off balance and resulted in decreased seawater pH in marine ecosystem, termed ocean acidification (OA). Anthropogenic OA is postulated to affect the physiology of many marine calcifying organisms. However, the susceptibility and metabolic pathways of change in most calcifying animals are still far from being well understood. In this work, the effects of exposure to elevated pCO(2) were characterized in gills and hepatopancreas of Crassostrea gigas using integrated proteomic and metabolomic approaches. Metabolic responses indicated that high CO2 exposure mainly caused disturbances in energy metabolism and osmotic regulation marked by differentially altered ATP, glucose, glycogen, amino acids and organic osmolytes in oysters, and the depletions of ATP in gills and the accumulations of ATP, glucose and glycogen in hepatopancreas accounted for the difference in energy distribution between these two tissues. Proteomic responses suggested that OA could not only affect energy and primary metabolisms, stress responses and calcium homeostasis in both tissues, but also influence the nucleotide metabolism in gills and cytoskeleton structure in hepatopancreas. This study demonstrated that the combination of proteomics and metabolomics could provide an insightful view into the effects of OA on oyster C. gigas. Biological significance The gradually increased atmospheric CO2 partial pressure (pCO(2)) has thrown the carbonate chemistry off balance and resulted in decreased seawater pH in marine ecosystem, termed ocean acidification (OA). Anthropogenic OA is postulated to affect the physiology of many marine calcifying organisms. However, the susceptibility and metabolic pathways of change in most calcifying animals are still far from being understood. To our knowledge, few studies have focused on the responses induced by pCO(2) at both protein and metabolite levels. The pacific oyster C. gigas, widely distributed throughout most of the world's oceans, is a model organism for marine environmental science. In the present study, an integrated metabolomic and proteomic approach was used to elucidate the effects of ocean acidification on Pacific oyster C. gigas, hopefully shedding light on the physiological responses of marine mollusk to the OA stress. (C) 2014 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods
研究领域[WOS]	National Natural Science Foundation of China [31172388] ; Key Research Program of the Chinese Academy of Sciences [KZZD-EW-14]
关键词[WOS]	LASER-DESORPTION/IONIZATION-TIME ; CLAM RUDITAPES-PHILIPPINARUM ; OCEAN ACIDIFICATION ; ARGININE KINASE ; TROPONIN-C ; TRYPANOSOMA-CRUZI ; OXIDATIVE STRESS ; PROTEIN ; GENE ; CARBON
收录类别	SCI
语种	英语
WOS记录号	WOS:000347021100007
公开日期	2015-08-04
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8677]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
推荐引用方式 GB/T 7714	Ji, Chenglong,Wang, Qing. Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure[J]. JOURNAL OF PROTEOMICS,2015,112:83-94.
APA	Ji, Chenglong,&Wang, Qing.(2015).Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure.JOURNAL OF PROTEOMICS,112,83-94.
MLA	Ji, Chenglong,et al."Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure".JOURNAL OF PROTEOMICS 112(2015):83-94.