Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)
	Wei, Lei 1; Wang, Qing1 ; Ning, Xuanxuan 2; Mu, Changkao 3; Wang, Chunlin 3; Cao, Ruiwen 1,4; Wu, Huifeng1 ; Cong, Ming1 ; Li, Fei1 ; Ji, Chenglong1
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
	2015-03-01
卷号	13 页码:16-23
关键词	Ocean acidification Proteomics Metabolomics Crassostrea gigas
ISSN号	1744-117X
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn
产权排序	[Wei, Lei; Wang, Qing; Cao, Ruiwen; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [Mu, Changkao; Wang, Chunlin] Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China; [Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
中文摘要	Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO(2) condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO(2) could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO(2) probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C.gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. (C) 2014 Elsevier Inc. All rights reserved.
英文摘要	Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO(2) condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO(2) could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO(2) probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C.gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. (C) 2014 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Genetics & Heredity
研究领域[WOS]	Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]	LASER-DESORPTION/IONIZATION-TIME ; CLAM RUDITAPES-PHILIPPINARUM ; EARTHWORM EISENIA-FOETIDA ; SMOOTH-MUSCLE MYOSIN ; OCEAN ACIDIFICATION ; MYTILUS-GALLOPROVINCIALIS ; SACCOSTREA-GLOMERATA ; 2,2',4,4'-TETRABROMODIPHENYL ETHER ; MALATE-DEHYDROGENASES ; METAL CONTAMINATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000351032100003
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8646]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China 3.Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wei, Lei,Wang, Qing,Ning, Xuanxuan,et al. Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2015,13:16-23.
APA	Wei, Lei.,Wang, Qing.,Ning, Xuanxuan.,Mu, Changkao.,Wang, Chunlin.,...&Zhao, Jianmin.(2015).Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,13,16-23.
MLA	Wei, Lei,et al."Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 13(2015):16-23.