A new structure-based classification of sulfide:quinone oxidoreductases

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	A new structure-based classification of sulfide:quinone oxidoreductases
	Marcia, Marco 2; Ermler, Ulrich 2; Peng, Guohong 1,2; Michel, Hartmut 2
刊名	PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
	2010-04-01
卷号	78 期号:5 页码:1073-1083
关键词	flavoproteins disulfide reductase superfamily structure-functional relationships Aquifex aeolicus Acidianus ambivalens monotopic membrane proteins flavin adenine dinucleotide sulfur polymerization structure-based sequence alignment
英文摘要	Sulfide:quinone oxidoreductases (SQR) are ubiquitous membrane-bound flavoproteins involved in sulfide detoxification, in sulfide-dependent energy conservation processes and potenatially in the homeostasis of the neurotransmitter sulfide. The first 2 structures of SQRs from the bacterium Aquifex aeolicus (Marcia et al., Proc Nad Acad Sci USA 2009; 106:96259630) and the archaeon Acidianus ambivalens (Brito et al., Biochemistry 2009; 48:5613-5622) were determined recently by Xray crystallography revealing unexpected differences in the active sites and in flavin adenine dinucleotide binding. Besides the reciprocal differences, they show a different conformation of the active site compared with another sulfide oxidizing enzyme, the flavocytochrome c-sulfide dehydrogenase (FCSD) from Allochromatium vinosum (protein data bank id: 1FCD). In addition to the new structural data, the number of available SQR-like protein sequences is continuously increasing (Pham et aL, Microbiology 2008; 154:3112-3121) and the SQR activity of new members of this protein family was recently proven too (Chan et al., J Bacteriol 2009; 191:1026-1034). In the light of the new data, here we revisit the previously proposed contradictory SQR classification and we define new structure-based sequence fingerprints that support a subdivision of the SQR family into six groups. Our report summarizes the state-of-art knowledge about SQRs and highlights the questions that still remain unanswered. Despite two decades of work already done on these enzymes, new and most exciting discoveries can be expected in the future.
收录类别	SCI
语种	英语
WOS记录号	WOS:000275639500001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/32767]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Max Planck Inst Biophys, Dept Mol Membrane Biol, D-60596 Frankfurt, Germany
推荐引用方式 GB/T 7714	Marcia, Marco,Ermler, Ulrich,Peng, Guohong,et al. A new structure-based classification of sulfide:quinone oxidoreductases[J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,2010,78(5):1073-1083.
APA	Marcia, Marco,Ermler, Ulrich,Peng, Guohong,&Michel, Hartmut.(2010).A new structure-based classification of sulfide:quinone oxidoreductases.PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,78(5),1073-1083.
MLA	Marcia, Marco,et al."A new structure-based classification of sulfide:quinone oxidoreductases".PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 78.5(2010):1073-1083.