Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis
	Meng, C. X.; Wei, W.; Su, Z-L ; Qin, S.
刊名	INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
	2006-10-01
卷号	43 期号:5 页码:284-288
关键词	Haematococcus pluvialis astaxanthin carotenoid hydroxylase cis-acting element promoter
英文摘要	Astaxanthin, a high-value ketocarotenoid is mainly used in fish aquaculture. It also has potential in human health due to its higher antioxidant capacity than beta-carotene and vitamin E. The unicellular green alga Haematococcus pluvialis is known to accumulate astaxanthin in response to environmental stresses, such as high light intensity and salt stress. Carotenoid hydroxylase plays a key role in astaxanthin biosynthesis in H. pleevialis. In this paper, we report the characterization of a promoter-like region (378 to -22 bp) of carotenoid hydroxylase gene by cloning, sequence analysis and functional verification of its 919 by 5'-flanking region in H. pluvialis. The 5'-flanking region was characterized using micro-particle bombardment method and transient expression of LacZ reporter gene. Results of sequence analysis showed that the 5'-flanking region might have putative cis-acting elements, such as ABA (abscisic acid)-responsive element (ABRE), C-repeat/dehydration responsive element (C-repeadDRE), ethylene-responsive element (ERE), heat-shock element (HSE), wound-responsive element (WUN-motif), gibberellin-responsive element (P-box), MYB-binding site (MBS) etc., except for typical TATA and CCAAT boxes. Results of 5' deletions construct and beta-galactosidase assays revealed that a highest promoter-like region might exist from -378 to -22 by and some negative regulatory elements might lie in the region from -919 to -378 bp. Results of site-directed mutagenesis of a putative C-repeat/DRE and an ABRE-like motif in the promoterlike region (-378 to -22 bp) indicated that the putative C-repeatJDRE and ABRE-like motif might be important for expression of carotenoid hydroxylase gene.
收录类别	SCI
语种	英语
WOS记录号	WOS:000241565800003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/32554]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Meng, C. X.,Wei, W.,Su, Z-L,et al. Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis[J]. INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,2006,43(5):284-288.
APA	Meng, C. X.,Wei, W.,Su, Z-L,&Qin, S..(2006).Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis.INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,43(5),284-288.
MLA	Meng, C. X.,et al."Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis".INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS 43.5(2006):284-288.