Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy
	Ye, Yansheng 1,2; Liu, Xiaoli 1; Xu, Guohua 1; Liu, Maili 1; Li, Conggang 1
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2015-04-27
卷号	54 期号:18 页码:5328-5330
关键词	calmodulin fluorine NMR spectroscopy protein conformation signal transduction
英文摘要	calmodulin (CaM) is essential to a variety of signal transduction pathways. Whether the transition in living cells is similar to that observed in buffer is not known. Here, we report the direct observation by F-19 NMR spectroscopy of the transition of the Ca2+-free and -bound forms in Xenopus laevis oocytes at different Ca2+ levels. We find that the Ca2+-bound CaM population increased greatly upon binding the target protein myosin light-chain kinase (MLCK) at the same Ca2+ level. Paramagnetic NMR spectroscopy was also exploited for the first time to obtain long-range structural constraints in cells. Our study shows that F-19 NMR spectroscopy can be used to obtain long-range structural constraints in living eukaryotic cells and paves the way for quantification of protein binding constants.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	MAGNETIC-RESONANCE-SPECTROSCOPY ; ESCHERICHIA-COLI ; PROTEIN INTERACTIONS ; IN-VIVO ; CELLS ; REVEALS ; KINASE ; CA2+ ; WEAK
收录类别	SCI
语种	英语
WOS记录号	WOS:000354191600007
公开日期	2015-07-28
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/4740]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ye, Yansheng,Liu, Xiaoli,Xu, Guohua,et al. Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015,54(18):5328-5330.
APA	Ye, Yansheng,Liu, Xiaoli,Xu, Guohua,Liu, Maili,&Li, Conggang.(2015).Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,54(18),5328-5330.
MLA	Ye, Yansheng,et al."Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 54.18(2015):5328-5330.