Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy | |
Ye, Yansheng1,2; Liu, Xiaoli1; Xu, Guohua1; Liu, Maili1; Li, Conggang1 | |
刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
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2015-04-27 | |
卷号 | 54期号:18页码:5328-5330 |
关键词 | calmodulin fluorine NMR spectroscopy protein conformation signal transduction |
英文摘要 | calmodulin (CaM) is essential to a variety of signal transduction pathways. Whether the transition in living cells is similar to that observed in buffer is not known. Here, we report the direct observation by F-19 NMR spectroscopy of the transition of the Ca2+-free and -bound forms in Xenopus laevis oocytes at different Ca2+ levels. We find that the Ca2+-bound CaM population increased greatly upon binding the target protein myosin light-chain kinase (MLCK) at the same Ca2+ level. Paramagnetic NMR spectroscopy was also exploited for the first time to obtain long-range structural constraints in cells. Our study shows that F-19 NMR spectroscopy can be used to obtain long-range structural constraints in living eukaryotic cells and paves the way for quantification of protein binding constants. |
WOS标题词 | Science & Technology ; Physical Sciences |
类目[WOS] | Chemistry, Multidisciplinary |
研究领域[WOS] | Chemistry |
关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; ESCHERICHIA-COLI ; PROTEIN INTERACTIONS ; IN-VIVO ; CELLS ; REVEALS ; KINASE ; CA2+ ; WEAK |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000354191600007 |
公开日期 | 2015-07-28 |
内容类型 | 期刊论文 |
源URL | [http://ir.wipm.ac.cn/handle/112942/4740] ![]() |
专题 | 武汉物理与数学研究所_磁共振基础研究部 |
作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Ye, Yansheng,Liu, Xiaoli,Xu, Guohua,et al. Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015,54(18):5328-5330. |
APA | Ye, Yansheng,Liu, Xiaoli,Xu, Guohua,Liu, Maili,&Li, Conggang.(2015).Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,54(18),5328-5330. |
MLA | Ye, Yansheng,et al."Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 54.18(2015):5328-5330. |
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