Probing methyl dynamics from C-13 autocorrelated and cross-correlated relaxation

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Probing methyl dynamics from C-13 autocorrelated and cross-correlated relaxation
	Zhang, X ; Sui, XG ; Yang, DW
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2006-04-19
卷号	128 期号:15 页码:5073-5081
英文摘要	An understanding of side-chain motions in protein is of great interest since side chains often play an important role in protein folding and intermolecular interactions. A novel method for measuring dipole-dipole cross-correlated relaxation in methyl groups of uniformly C-13-labeled proteins without deuteration has been developed by our group. The excellent agreement between dynamic parameters of methyl groups in ubiquitin obtained from the cross-correlated relaxation and C-13 spin-lattice relaxation and those derived previously from 2 H relaxation data demonstrates the reliability of the method. This method was applied to the study of side-chain dynamics of human intestinal fatty acid binding protein (IFABP) with and without its ligand. Binding of oleic acid to the protein results in decreased mobility of most of the methyl groups in the binding region, whereas no significant change in mobility was observed for methyl groups in the nonbinding region.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	SIDE-CHAIN DYNAMICS ; ACID-BINDING-PROTEIN ; N-15 NMR RELAXATION ; MODEL-FREE APPROACH ; MAGNETIC-RESONANCE RELAXATION ; DEUTERIUM SPIN PROBES ; TERMINAL SH3 DOMAIN ; FATTY-ACID ; STAPHYLOCOCCAL NUCLEASE ; TRANSVERSE RELAXATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000236917200038
公开日期	2015-07-28
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/4676]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore 2.Chinese Acad Sci, State Key Lab Magnet Resonance & Mol & Atom Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 3.Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
推荐引用方式 GB/T 7714	Zhang, X,Sui, XG,Yang, DW. Probing methyl dynamics from C-13 autocorrelated and cross-correlated relaxation[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2006,128(15):5073-5081.
APA	Zhang, X,Sui, XG,&Yang, DW.(2006).Probing methyl dynamics from C-13 autocorrelated and cross-correlated relaxation.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,128(15),5073-5081.
MLA	Zhang, X,et al."Probing methyl dynamics from C-13 autocorrelated and cross-correlated relaxation".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 128.15(2006):5073-5081.