Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing

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	Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing
	Sun YN; Chen XD(陈晓东); Zhou XG; Zhu JB; Yu YD
刊名	LAB ON A CHIP
	2015
通讯作者邮箱	yudeyu@semi.ac.cn
卷号	15 期号:11 页码:2429-2436
ISSN号	1473-0197
通讯作者	Yu, YD (reprint author), Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, POB 912, Beijing 100083, Peoples R China.
产权排序	[Sun, Yingnan; Zhou, Xiaoguang; Yu, Yude] Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, Beijing 100083, Peoples R China; [Chen, Xiaodong] Chinese Acad Sci, State Key Lab Nonlinear Mech, Inst Mech, Beijing 100190, Peoples R China; [Zhu, Jinbiao] Chinese Acad Sci, Inst Elect, Beijing 100190, Peoples R China
中文摘要	In recent years, inkjet printing, as a new method to fabricate microdroplet microarrays, has been increasingly applied in the field of biochemical diagnostics. To further improve the general applicability of the inkjet printing technology in fabricating biochemical chips, in this work, we introduce a model to describe the multiple injection procedure implemented by the inkjet printing approach, with experimental verification. The multiple injection model demonstrates a new sequential inkjet printing method that generates picoliter-scale multicomponent droplet-in-oil arrays via multistep printing on uniform planar substrates. Based on our previous work on double-inkjet printing, this technique adapts the piezoelectric inkjet printing technology to fabricate an oil droplet array, into which multiple precise injections of secondary droplets with different compositions and volumes can be automatically printed in the required sequence, simultaneously addressing the evaporation issues associated with printing picoliter droplets without external assistance. In this paper, we first describe the theory and characterize the model, which account for the basic principles of sequential inkjet printing, as well as validate the design in terms of multiple injections, droplet fusion, and rapid mixing. The feasibility and effectiveness of the method are also demonstrated in a dual fluorescence assay and a beta-galactosidase enzyme inhibition assay. We believe that applying the sequential inkjet printing methodology in existing inkjet printing devices will enhance their use as universal diagnostic tools as well as accelerate the adoption of inkjet printing in multistep screening experiments.
分类号	一类
类目[WOS]	Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
收录类别	SCI
原文出处	http://dx.doi.org/10.1039/c5lc00356c
语种	英语
WOS记录号	WOS:000354963400010
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/54965]
专题	力学研究所_非线性力学国家重点实验室
推荐引用方式 GB/T 7714	Sun YN,Chen XD,Zhou XG,et al. Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing[J]. LAB ON A CHIP,2015,15(11):2429-2436.
APA	Sun YN,陈晓东,Zhou XG,Zhu JB,&Yu YD.(2015).Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing.LAB ON A CHIP,15(11),2429-2436.
MLA	Sun YN,et al."Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing".LAB ON A CHIP 15.11(2015):2429-2436.