A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system

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	A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system
	Li, Hui1,2 ; Zhang, Huan 1; Jiang, Shuai 1; Wang, Weilin1,2 ; Xin, Lusheng1,2 ; Wang, Hao 1; Wang, Lingling 1; Song, Linsheng 1,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-06-01
卷号	44 期号:2 页码:566-575
关键词	Crassostrea gigas C-type lectin Innate immunity PRR Opsonin Growth suppression activity Complement system
英文摘要	C-type lectins (CTLs), serving as pattern recognition receptors (PRRs), are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that participate in nonself-recognition and pathogen elimination. In the present study, a single carbohydrate-recognition domain (CRD) CTL was identified from oyster Crassostrea gigas (designated as CgCLec-2). There was only one CRD within the deduced amino acid sequence of CgCLec-2 consisting of 129 amino acid residues. A conserved EPN (Glu(246)-Pro(247)-Asn(248)) motif was found in Ca2+-binding site 2 of CgCLec-2. The CgCLec-2 mRNA could be detected in all the examined tissues at different expression levels in oysters. The mRNA expression of CgCLec-2 in hemocytes was up-regulated significantly at 6 h post Vibrio splendidus challenge. The recombinant CgCLec-2 (rCgCLec-2) could bind various Pathogen-Associated Molecular Patterns (PAMPs), including lipopolysaccharide, mannan and peptidoglycan, and displayed strong binding abilities to Vibrio anguillarum, V. splendidus and Yarrowiali polytica and week binding ability to Staphylococcus aureus. It could also enhance the phagocytic activity of oyster hemocytes to V. splendidus and exhibited growth suppression activity against gram-positive bacteria S. aureus but no effect on gram-negative bacteria V. splendidus. Furthermore, the interaction between rCgCLec-2 and rCgMASPL-1 was confirmed by GST Pull down. The results suggested that CgCLec-2 served as not only a PRR in immune recognition but also a regulatory factor in pathogen elimination, and played a potential role in the activation of complement system. (c) 2015 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000354583800021
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/26971]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Ocean Univ, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Li, Hui,Zhang, Huan,Jiang, Shuai,et al. A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(2):566-575.
APA	Li, Hui.,Zhang, Huan.,Jiang, Shuai.,Wang, Weilin.,Xin, Lusheng.,...&Song, Linsheng.(2015).A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system.FISH & SHELLFISH IMMUNOLOGY,44(2),566-575.
MLA	Li, Hui,et al."A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system".FISH & SHELLFISH IMMUNOLOGY 44.2(2015):566-575.