CcpA-Mediated Enhancement of Sugar and Amino Acid Metabolism in Lysinibacillus sphaericus by NMR-Based Metabolomics

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	CcpA-Mediated Enhancement of Sugar and Amino Acid Metabolism in Lysinibacillus sphaericus by NMR-Based Metabolomics
	Li, Jing 1,3; Huang, Chongyang 2,3; Zheng, Dasheng 1; Wang, Yulan 2; Yuan, Zhiming 1
刊名	JOURNAL OF PROTEOME RESEARCH
	2012-09-01
卷号	11 期号:9 页码:4654-4661
关键词	Lysinibacillus sphaericus catabolite control protein A deficient growth metabolome metabolic networks
英文摘要	Lysinibacillus sphaericus is a bacterium incapable of metabolizing sugars with the sole exception of N-acetylglucosamine. To unravel the regulatory role of catabolite control protein A (CcpA) in the sugar metabolism of L. sphaericus, a ccpA deficient mutant was constructed by homologous recombination. The mutant showed growth deficiency and a low efficiency of carbon and energy utilization. NMR spectroscopy in combination with multivariate data analysis revealed that the metabolome of L. sphaericus was dominated by 25 metabolites mainly including amino acids, carbohydrate derivatives and organic acids, and that the mutation of the ccpA gene caused significant reduction of leucine, valine, alanine, threonine, glutamate, lysine, D-ornithine, tyrosine, uridine 5'-diphospho-N-acetlyglucosamine formate, fumarate, phenylalanine, aspartate, asparagine, and acetate but elevation of ribose-5-phosphate, and uracil. Furthermore, the networks of CcpA-mediated regulation based on the metabolome were constructed by arrangement of significantly decreasing or increasing metabolites. The network map suggests CcpA regulates and promotes sugar and amino acid metabolism of L. sphaericus.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	GRAM-POSITIVE BACTERIA ; SUBTILIS GNT OPERON ; BACILLUS-SUBTILIS ; CATABOLITE REPRESSION ; PHOSPHOTRANSFERASE SYSTEM ; HPR KINASE/PHOSPHORYLASE ; PROTEOME ANALYSIS ; GENE-EXPRESSION ; SENSOR ENZYME ; CARBON
收录类别	SCI
语种	英语
WOS记录号	WOS:000308389900023
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1729]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Appl & Environm Microbiol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing,Huang, Chongyang,Zheng, Dasheng,et al. CcpA-Mediated Enhancement of Sugar and Amino Acid Metabolism in Lysinibacillus sphaericus by NMR-Based Metabolomics[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(9):4654-4661.
APA	Li, Jing,Huang, Chongyang,Zheng, Dasheng,Wang, Yulan,&Yuan, Zhiming.(2012).CcpA-Mediated Enhancement of Sugar and Amino Acid Metabolism in Lysinibacillus sphaericus by NMR-Based Metabolomics.JOURNAL OF PROTEOME RESEARCH,11(9),4654-4661.
MLA	Li, Jing,et al."CcpA-Mediated Enhancement of Sugar and Amino Acid Metabolism in Lysinibacillus sphaericus by NMR-Based Metabolomics".JOURNAL OF PROTEOME RESEARCH 11.9(2012):4654-4661.