NMR analysis of the rat neurochemical changes induced by middle cerebral artery occlusion

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	NMR analysis of the rat neurochemical changes induced by middle cerebral artery occlusion
	Yang, Mingxing 1; Wang, Shu 2; Hao, Fuhua1 ; Li, Yajie 3; Tang, Huiru 1; Shi, Xuemin
刊名	TALANTA
	2012-01-15
卷号	88 页码:136-144
关键词	Metabonomics Focal cerebral ischemia MCAO Pattern recognition NMR spectroscopy Neurochemistry
英文摘要	Stroke is a leading cause of death and disability, affecting millions of people worldwide with almost 80% of them as ischemic stroke and understanding the multiple mechanisms underlying cerebral ischemia is essential for development of effective treatments. To understand metabolic changes induced by focal brain ischemia, we conducted a comparative analysis of metabolic composition of cerebral tissue from rats with sham-operation and middle cerebral artery occlusion (MCAO) using high-resolution nuclear magnetic resonance (NMR) spectroscopy. More than 40 metabolites were assigned including organic acids, amino acids, carbohydrates, choline, pyrimidine and purine metabolites. Our results showed that MCAO led to significant level decreases for glutamate, glutamine, aspartate, gamma-aminobutyrate (GABA), taurine, malate, fumarate, acetate, phosphocreatine, and purine and pyrimidine metabolites such as inosine, hypoxanthine, xanthine, uracil and UDP/UTP, together with significant level increases for glucose in focal brain tissue extracts. This demonstrated that experimental ischemic stroke in rats caused extensive perturbation in tricarboxylic acid cycle. GABA shunt, and metabolisms of choline and nucleic acids. These findings provided essential information for our understandings of MCAO-caused biochemical alterations and demonstrated the metabolite composition analysis as a useful tool for understanding the neurochemistry of stroke. (C) 2011 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	MAGNETIC-RESONANCE SPECTROSCOPY ; PRINCIPAL COMPONENT ANALYSIS ; METABOLIC-RESPONSES ; ISCHEMIC-STROKE ; BRAIN ; METABONOMICS ; ADENOSINE ; H-1 ; PLS ; HOMEOSTASIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000301159400016
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1713]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Tianjin Univ Tradit Chinese Med, Tianjin Key Lab Acupuncture & Moxibust, Affiliated Hosp 1, Tianjin 300193, Peoples R China 3.Capital Med Univ, Beijing Chao Yang Hosp, Dept Chinese Med, Beijing 100020, Peoples R China
推荐引用方式 GB/T 7714	Yang, Mingxing,Wang, Shu,Hao, Fuhua,et al. NMR analysis of the rat neurochemical changes induced by middle cerebral artery occlusion[J]. TALANTA,2012,88:136-144.
APA	Yang, Mingxing,Wang, Shu,Hao, Fuhua,Li, Yajie,Tang, Huiru,&Shi, Xuemin.(2012).NMR analysis of the rat neurochemical changes induced by middle cerebral artery occlusion.TALANTA,88,136-144.
MLA	Yang, Mingxing,et al."NMR analysis of the rat neurochemical changes induced by middle cerebral artery occlusion".TALANTA 88(2012):136-144.