Age-Related Topographical Metabolic Signatures for the Rat Gastrointestinal Contents

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	Age-Related Topographical Metabolic Signatures for the Rat Gastrointestinal Contents
	Tian, Yuan 1,2; Zhang, Limin 1; Wang, Yulan 1; Tang, Huiru 1
刊名	JOURNAL OF PROTEOME RESEARCH
	2012-02-01
卷号	11 期号:2 页码:1397-1411
关键词	gastrointestinal content feces gut microbiota H-1 NMR spectroscopy metabonomics multivariate statistical analysis
英文摘要	Symbiotic gut microbiota is essential for mammalian physiology and analyzing the metabolite compositions of gastrointestinal contents is vital for understanding the microbiome-host interactions. To understand the developmental dependence of the topographical metabolic signatures for the rat gastrointestinal contents, we systematically characterized the metabolite compositional variations of the contents in rat jejunum, ileum, cecum, and colon for two age-groups using H-1 NMR spectroscopy and multivariate analysis. Significant topographical metabolic variations were present for the jejunal, ileal, cecal, colonic contents, and feces, reflecting the absorption functions for each intestinal region and the gut microbiota therein. The concentrations of amino acids, lactate, creatine, choline, bile acids, uracil and urocanate decreased drastically from jejunal to ileal contents followed with steady decreases from cecal content to feces. Short-chain fatty acids (SCFAs) and arabinoxylan-related carbohydrates had highest levels in cecal content and feces, respectively. Such topographical metabolic signatures for the intestinal contents varied with animal age highlighted by the level changes for lactate, choline, taurine, amino acids, carbohydrates, keto-acids, and SCFAs. These findings provided essential information for the topographical metabolic variations in the gastrointestinal tract and demonstrated metabolic profiling as a useful approach for understanding host-microbiome interactions and functional status of the gastrointestinal regions.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	TOTAL CORRELATION SPECTROSCOPY ; SPINNING H-1-NMR SPECTROSCOPY ; INFLAMMATORY-BOWEL-DISEASE ; IN-VITRO FERMENTATION ; INTESTINAL BACTERIA ; GUT MICROBIOTA ; TAURINE TRANSPORT ; ABSORPTION ; TRACT ; NMR
收录类别	SCI
语种	英语
WOS记录号	WOS:000300458300076
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1707]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Tian, Yuan,Zhang, Limin,Wang, Yulan,et al. Age-Related Topographical Metabolic Signatures for the Rat Gastrointestinal Contents[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(2):1397-1411.
APA	Tian, Yuan,Zhang, Limin,Wang, Yulan,&Tang, Huiru.(2012).Age-Related Topographical Metabolic Signatures for the Rat Gastrointestinal Contents.JOURNAL OF PROTEOME RESEARCH,11(2),1397-1411.
MLA	Tian, Yuan,et al."Age-Related Topographical Metabolic Signatures for the Rat Gastrointestinal Contents".JOURNAL OF PROTEOME RESEARCH 11.2(2012):1397-1411.