Dynamic metabolomic responses of Escherichia coli to nicotine stress

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	Dynamic metabolomic responses of Escherichia coli to nicotine stress
	Ding, Lijian 1; Chen, Juanjuan 1; Zou, Jianding 2; Zhang, Limin 3; Ye, Yangfang 1
刊名	CANADIAN JOURNAL OF MICROBIOLOGY
	2014-08-01
卷号	60 期号:8 页码:547-556
关键词	nicotine Escherichia coli metabolomics nuclear magnetic resonance (NMR) multivariate data analysis
英文摘要	Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolomics in combination with multivariate data analysis methods to comprehensively analyze the metabolic changes in nicotine-treated Escherichia coli. Our results showed that nicotine caused the changes of energy-related metabolism that we believe are due to enhanced glycolysis and mixed acid fermentation as well as inhibited tricarboxylic acid cycle activity. Furthermore, nicotine resulted in the alteration of choline metabolism with a decreased synthesis of betaine but an increased production of dimethylamine. Moreover, nicotine caused a decrease in amino acid concentration and an alteration of nucleotide synthesis. We hypothesize that these changes caused the decrease in bacterial cell density observed in the experiment. These findings provide a comprehensive insight into the metabolic response of E. coli to nicotine stress. Our study highlights the value of metabolomics in elucidating the metabolic mechanisms of nicotine action.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
关键词[WOS]	GLYCINE BETAINE PATHWAY ; NMR-BASED METABOLOMICS ; OXIDATIVE STRESS ; CANCER-CELLS ; EXPRESSION ; PROTEINS ; INDICATORS ; APOPTOSIS ; RELEASE ; GROWTH
收录类别	SCI
语种	英语
WOS记录号	WOS:000341918300007
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1352]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China 2.Cixi Peoples Hosp, Cixi 315300, Zhejiang, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Ding, Lijian,Chen, Juanjuan,Zou, Jianding,et al. Dynamic metabolomic responses of Escherichia coli to nicotine stress[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2014,60(8):547-556.
APA	Ding, Lijian,Chen, Juanjuan,Zou, Jianding,Zhang, Limin,&Ye, Yangfang.(2014).Dynamic metabolomic responses of Escherichia coli to nicotine stress.CANADIAN JOURNAL OF MICROBIOLOGY,60(8),547-556.
MLA	Ding, Lijian,et al."Dynamic metabolomic responses of Escherichia coli to nicotine stress".CANADIAN JOURNAL OF MICROBIOLOGY 60.8(2014):547-556.