Systemic Responses of Mice to Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis Using H-1 NMR Spectroscopy

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	Systemic Responses of Mice to Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis Using H-1 NMR Spectroscopy
	Dong, Fangcong 1,2; Zhang, Lulu 1,2; Hao, Fuhua1 ; Tang, Huiru 1; Wang, Yulan 1,3
刊名	JOURNAL OF PROTEOME RESEARCH
	2013-06-01
卷号	12 期号:6 页码:2958-2966
关键词	metabonomics inflammatory bowel diseases ulcerative colitis nuclear magnetic resonance spectroscopy dextran sulfate sodium mice
英文摘要	The interplay between genetic mutation and environmental factors is believed to contribute to the etiology of inflammatory bowel disease (IBD). While focused attention has been paid to the aforementioned research, time-specific and organ-specific metabolic changes associated with IBD are still lacking. Here, we induced acute ulcerative colitis in mice by providing water containing 3% dextran sulfate sodium (DSS) for 7 days and investigated the metabolic changes of plasma, urine, and a range of biological tissues by employing a H-1 nuclear magnetic resonance (NMR)-based metabonomics approach with complementary information on serum clinical chemistry and histopathology. We found that DSS-induced acute ulcerative colitis leads to significant elevations in the levels of amino acids in plasma and decreased levels in the membrane-related metabolites and a range of nucleotides, nucleobases, and nucleosides in the colon. In addition, acute-colitis-induced elevations in the levels of nucleotides in the liver were observed, accompanied by reduced levels of glucose. DSS-induced acute colitis also resulted in increased levels of oxidized glutathione and attenuated levels of taurine in the spleen. Furthermore, acute colitis resulted in depletion in the levels of gut microbial cometabolites in urine along with an increase in citric acid cycle intermediates. These findings suggest that DSS-induced acute colitis causes a disturbance of lipid and energy metabolism, damage to the colon and liver, a promoted antioxidative and anti-inflammatory response, and perturbed gut microbiotal communities. The information obtained here provided details of the time-dependent and holistic metabolic changes in the development of the DSS-induced acute ulcerative colitis, which could be useful in discovery of novel therapeutic targets for management of IBD.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	INFLAMMATORY-BOWEL-DISEASE ; BIOMARKER IDENTIFICATION ; METABOLIC-RESPONSES ; CROHNS-DISEASE ; METABONOMICS ; SUSCEPTIBILITY ; BACTERIA ; MODEL ; PROLIFERATION ; INDUCTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000320298600053
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/897]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Dong, Fangcong,Zhang, Lulu,Hao, Fuhua,et al. Systemic Responses of Mice to Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis Using H-1 NMR Spectroscopy[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(6):2958-2966.
APA	Dong, Fangcong,Zhang, Lulu,Hao, Fuhua,Tang, Huiru,&Wang, Yulan.(2013).Systemic Responses of Mice to Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis Using H-1 NMR Spectroscopy.JOURNAL OF PROTEOME RESEARCH,12(6),2958-2966.
MLA	Dong, Fangcong,et al."Systemic Responses of Mice to Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis Using H-1 NMR Spectroscopy".JOURNAL OF PROTEOME RESEARCH 12.6(2013):2958-2966.