H-1-NMR Spectroscopy Revealed Mycobacterium tuberculosis Caused Abnormal Serum Metabolic Profile of Cattle

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	H-1-NMR Spectroscopy Revealed Mycobacterium tuberculosis Caused Abnormal Serum Metabolic Profile of Cattle
	Chen, Yingyu 1,2; Wu, Junfang 4; Tu, Lingling 1,5; Xiong, Xuekai 1,3; Hu, Xidan 6; Huang, Jiong 6; Xu, Zhiguang 6; Zhang, Xiansong 1,3; Hu, Changmin 3; Hu, Xueying 3
刊名	PLOS ONE
	2013-09-30
卷号	8 期号:9
英文摘要	To re-evaluate virulence of Mycobacterium tuberculosis (M. tb) in cattle, we experimentally infected calves with M. tb and Mycobacterium bovisvia intratracheal injection at a dose of 2.0x10(7) CFU and observed the animals for 33 weeks. The intradermal tuberculin test and IFN-gamma in vitro release assay showed that both M. tb and M. bovis induced similar responses. Immunohistochemical staining of pulmonary lymph nodes indicated that the antigen MPB83 of both M. tb and M. bovis were similarly distributed in the tissue samples. Histological examinations showed all of the infected groups exhibited neutrophil infiltration to similar extents. Although the infected cattle did not develop granulomatous inflammation, the metabolic profiles changed significantly, which were characterized by a change in energy production pathways and increased concentrations of N-acetyl glycoproteins. Glycolysis was induced in the infected cattle by decreased glucose and increased lactate content, and enhanced fatty acid beta-oxidation was induced by decreased TG content, and decreased gluconeogenesis indicated by the decreased concentration of glucogenic and ketogenic amino acids promoted utilization of substances other than glucose as energy sources. In addition, an increase in acute phase reactive serum glycoproteins, together with neutrophil infiltration and increased of IL-1 beta production indicated an early inflammatory response before granuloma formation. In conclusion, this study indicated that both M. tb and M. bovis were virulent to cattle. Therefore, it is likely that cattle with M. tb infections would be critical to tuberculosis transmission from cattle to humans. Nuclear magnetic resonance was demonstrated to be an efficient method to systematically evaluate M. tb and M. bovi sinfection in cattle.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	HUMAN-BLOOD-PLASMA ; MOLECULAR CHARACTERIZATION ; BIOMARKER DISCOVERY ; BOVINE TUBERCULOSIS ; INFECTION ; HOST ; H-1 ; TRANSMISSION ; METABONOMICS ; MARKER
收录类别	SCI
语种	英语
WOS记录号	WOS:000325423500025
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/856]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Huazhong Agr Univ, Natl Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China 2.Huazhong Agr Univ, Coll Anim Sci, Wuhan, Hubei, Peoples R China 3.Huazhong Agr Univ, Coll Vet Med, Wuhan, Hubei, Peoples R China 4.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan, Hubei, Peoples R China 5.Wuhan Keqian Anim Biol Prod Co Ltd, Wuhan, Hubei, Peoples R China 6.Xinjiang Acad Anim Husb & Vet Inst, Urumqi, Xinjiang, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yingyu,Wu, Junfang,Tu, Lingling,et al. H-1-NMR Spectroscopy Revealed Mycobacterium tuberculosis Caused Abnormal Serum Metabolic Profile of Cattle[J]. PLOS ONE,2013,8(9).
APA	Chen, Yingyu.,Wu, Junfang.,Tu, Lingling.,Xiong, Xuekai.,Hu, Xidan.,...&Chen, Huanchun.(2013).H-1-NMR Spectroscopy Revealed Mycobacterium tuberculosis Caused Abnormal Serum Metabolic Profile of Cattle.PLOS ONE,8(9).
MLA	Chen, Yingyu,et al."H-1-NMR Spectroscopy Revealed Mycobacterium tuberculosis Caused Abnormal Serum Metabolic Profile of Cattle".PLOS ONE 8.9(2013).