Concentration-Dependent Aggregation of CHAPS Investigated by NMR Spectroscopy

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Concentration-Dependent Aggregation of CHAPS Investigated by NMR Spectroscopy
	Qin, Xianguo 2,3; Liu, Maili 2; Yang, Daiwen 1; Zhang, Xu 2
刊名	JOURNAL OF PHYSICAL CHEMISTRY B
	2010-03-25
卷号	114 期号:11 页码:3863-3868
产权排序	第二
英文摘要	CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) is a zwitterionic surfactant, which has been extensively used in various biological fields. Ill the present work, the concentration-dependent aggregation Of the surfactant in deuterium Oxide solution was investigated by NMR spectroscopy. We have found that two break points exist oil the basis of the NMR parameters such as chemical shift difference (M), self-diffusion coefficient (D), and relaxation rates (R(1), R(2)). The first break point corresponds to the widely accepted normal critical micelle concentration (cmc). The second one is Unexpected and ascribed to the second cmc, indicating that there is another type of micelle at higher concentrations. Further analysis Using I D selective NOESY with spin-diffusion quenching reveals that at the concentration above the second cmc CHAPS may form a two-layer spherical structure of micelles, with the aliphatic groups of CHAPS molecules in the inner layer interact with the steroid groups ill the outer layer. The existence of two types of micelles has also been supported by our TEM experiment. The dependence of CHAPS micelle size oil concentration explains why some proteins are soluble and stable only at concentrations above the second cmc. Therefore, our finding provides a basis For optimizing CHAPS concentration in functional and structural studies of membrane proteins.
学科主题	波谱分析
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Physical
研究领域[WOS]	Chemistry
关键词[WOS]	ZWITTERIONIC DETERGENT ; MEMBRANE-PROTEINS ; MICELLE FORMATION ; SOLUBILIZATION ; DIFFUSION ; MICELLIZATION ; FLUOROCARBON ; SURFACTANTS ; SCATTERING ; MIXTURES
收录类别	SCI
语种	英语
WOS记录号	WOS:000275710400017
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1970]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore 2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan 430071, Peoples R China 3.Huazhong Univ Sci & Technol, Sch Phys, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Qin, Xianguo,Liu, Maili,Yang, Daiwen,et al. Concentration-Dependent Aggregation of CHAPS Investigated by NMR Spectroscopy[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2010,114(11):3863-3868.
APA	Qin, Xianguo,Liu, Maili,Yang, Daiwen,&Zhang, Xu.(2010).Concentration-Dependent Aggregation of CHAPS Investigated by NMR Spectroscopy.JOURNAL OF PHYSICAL CHEMISTRY B,114(11),3863-3868.
MLA	Qin, Xianguo,et al."Concentration-Dependent Aggregation of CHAPS Investigated by NMR Spectroscopy".JOURNAL OF PHYSICAL CHEMISTRY B 114.11(2010):3863-3868.