Systems Responses of Rats to Aflatoxin B1 Exposure Revealed with Metabonomic Changes in Multiple Biological Matrices

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Systems Responses of Rats to Aflatoxin B1 Exposure Revealed with Metabonomic Changes in Multiple Biological Matrices
	Zhang, Limin 1; Ye, Yangfang 1,2; An, Yanpeng 1; Tian, Yuan 1,3; Wang, Yulan 1; Tang, Huiru 1
刊名	JOURNAL OF PROTEOME RESEARCH
	2011-02-01
卷号	10 期号:2 页码:614-623
关键词	hepatotoxicology metabonomics aflatoxin B1 nuclear magnetic resonance (NMR)
产权排序	第一
英文摘要	Exposure to aflatoxins causes liver fibrosis and hepatocellular carcinoma posing a significant health risk for human populations and livestock. To understand the mammalian systems responses to aflatoxin-B1 (AFB1) exposure, we analyzed the AFB1-induced metabonomic changes in multiple biological matrices (plasma, urine, and liver) of rats using (1)H NMR spectroscopy together with clinical biochemistry and histopathologic assessments. We found that AFB1 exposure caused significant elevation of glucose, amino acids, and choline metabolites (choline, phosphocholine, and glycerophosphocholine) in plasma but reduction of plasma lipids. AFB1 also induced elevation of liver lipids, amino acids (tyrosine, histidine, phenylalanine, leucine, isoleucine, and valine), choline, and nucleic acid metabolites (inosine, adenosine, and uridine) together with reduction of hepatic glycogen and glucose. AFB1 further caused decreases in urinary TCA cycle intermediates (2-oxoglutarate and citrate) and elevation of gut microbiota cometabolites (phenylacetylglycine and hippurate). These indicated that AFB1 exposure caused hepatic steatosis accompanied with widespread metabolic changes including lipid and cell membrane metabolisms, protein biosynthesis, glycolysis, TCA cycle, and gut microbiota functions. This implied that AFB1 exposure probably caused oxidative-stress-mediated impairments of mitochondria functions. These findings provide an overview of biochemical consequences of AFB1 exposure and comprehensive insights into the metabolic aspects of AFB1-induced hepatotoxicity in rats.
学科主题	生物学
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	MAGNETIC-RESONANCE-SPECTROSCOPY ; HEPATOCELLULAR-CARCINOMA ; CARBON-TETRACHLORIDE ; GLYCINE CONJUGATION ; BLOOD-PLASMA ; IN-VIVO ; LIVER ; TOXICITY ; ACID ; NMR
收录类别	SCI
语种	英语
WOS记录号	WOS:000286868500021
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1870]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Limin,Ye, Yangfang,An, Yanpeng,et al. Systems Responses of Rats to Aflatoxin B1 Exposure Revealed with Metabonomic Changes in Multiple Biological Matrices[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(2):614-623.
APA	Zhang, Limin,Ye, Yangfang,An, Yanpeng,Tian, Yuan,Wang, Yulan,&Tang, Huiru.(2011).Systems Responses of Rats to Aflatoxin B1 Exposure Revealed with Metabonomic Changes in Multiple Biological Matrices.JOURNAL OF PROTEOME RESEARCH,10(2),614-623.
MLA	Zhang, Limin,et al."Systems Responses of Rats to Aflatoxin B1 Exposure Revealed with Metabonomic Changes in Multiple Biological Matrices".JOURNAL OF PROTEOME RESEARCH 10.2(2011):614-623.