Dynamic Metabonomic Responses of Tobacco (Nicotiana tabacum) Plants to Salt Stress

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Dynamic Metabonomic Responses of Tobacco (Nicotiana tabacum) Plants to Salt Stress
	Zhang, Jingtao 2,3; Zhang, Yong 1,3; Du, Yuanyuan 2,3; Chen, Shiyun 1; Tang, Huiru 2
刊名	JOURNAL OF PROTEOME RESEARCH
	2011-04-01
卷号	10 期号:4 页码:1904-1914
关键词	metabonomics salinity tobacco plants NMR multivariate data analysis
通讯作者	唐惠儒
产权排序	第一
英文摘要	Metabolic responses are important for plant adaptation to osmotic stresses. To understand the dosage and duration dependence of salinity effects on plant metabolisms, we analyzed the metabonome of tobacco plants and its dynamic responses to salt treatments using NMR spectroscopy in combination with multivariate data analysis. Our results showed that the tobacco metabonome was dominated by 40 metabolites including organic acids/bases, amino acids, carbohydrates and choline, pyrimidine, and purine metabolites. A dynamic trajectory was clearly observable for the tobacco metabonomic responses to the dosage of salinity. Short-term low-dose salt stress (50 mM NaCl, 1 day) caused metabolic shifts toward gluconeogenesis with depletion of pyrimidine and purine metabolites. Prolonged salinity with high-dose salt (500 mM NaCl) induced progressive accumulation of osmolytes, such as proline and myo-inositol, and changes in GABA shunt. Such treatments also promoted the shikimate-mediated secondary metabolisms with enhanced biosynthesis of aromatic amino acids. Therefore, salinity caused systems alterations in widespread metabolic networks involving transamination, TCA cycle, gluconeogenesis/glycolysis, glutamate-mediated proline biosynthesis, shikimate-mediated secondary metabolisms, and the metabolisms of choline, pyrimidine, and purine. These findings provided new insights for the tobacco metabolic adaptation to salinity and demonstrated the NMR-based metabonomics as a powerful approach for understanding the osmotic effects on plant biochemistry.
学科主题	波谱分析
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	PROLINE ACCUMULATION ; TRANSGENIC TOBACCO ; SALINITY STRESS ; WILD-TYPE ; ARABIDOPSIS-THALIANA ; METABOLITE PROFILES ; PROTEOMIC ANALYSIS ; ABIOTIC STRESS ; TOLERANCE ; NMR
收录类别	SCI
语种	英语
WOS记录号	WOS:000288924000043
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1801]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jingtao,Zhang, Yong,Du, Yuanyuan,et al. Dynamic Metabonomic Responses of Tobacco (Nicotiana tabacum) Plants to Salt Stress[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(4):1904-1914.
APA	Zhang, Jingtao,Zhang, Yong,Du, Yuanyuan,Chen, Shiyun,&Tang, Huiru.(2011).Dynamic Metabonomic Responses of Tobacco (Nicotiana tabacum) Plants to Salt Stress.JOURNAL OF PROTEOME RESEARCH,10(4),1904-1914.
MLA	Zhang, Jingtao,et al."Dynamic Metabonomic Responses of Tobacco (Nicotiana tabacum) Plants to Salt Stress".JOURNAL OF PROTEOME RESEARCH 10.4(2011):1904-1914.