Combined Metabonomic and Quantitative Real-Time PCR Analyses Reveal Systems Metabolic Changes of Fusarium graminearum Induced by Tri5 Gene Deletion

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Combined Metabonomic and Quantitative Real-Time PCR Analyses Reveal Systems Metabolic Changes of Fusarium graminearum Induced by Tri5 Gene Deletion
	Chen, Fangfang 2; Zhang, Jingtao 1,3; Song, Xiushi 2; Yang, Jian 2; Li, Heping 2; Tang, Huiru 1; Liao, Yu-Cai 2,4,5
刊名	JOURNAL OF PROTEOME RESEARCH
	2011-05-01
卷号	10 期号:5 页码:2273-2285
关键词	(1)H NMR Tri5 gene metabonomics Fusarium graminearum quantitative real-time PCR
通讯作者	Liao,YC
产权排序	第二
英文摘要	Fusarium graminearum (FG) is a serious plant pathogen causing huge losses in global production of wheat and other cereals. Tri5-gene encoded trichodiene synthase is the first key enzyme for biosynthesis of trichothecene mycotoxins in FG. To further our understandings of FG metabolism which is essential for developing novel strategies for controlling FG, we conducted a comprehensive investigation on the metabolic changes caused by Tri5-deletion by comparing metabolic differences between the wild-type FG5035 and an FG strain, Tri5(-), with Tri5 deleted. NMR methods identified more than 50 assigned fungal metabolites. Combined metabonomic and quantitative RT-PCR (qRT-PCR) analyses revealed that Tri5 deletion caused significant and comprehensive metabolic changes for FG apart from mycotoxin biosynthesis. These changes involved both carbon and nitrogen metabolisms including alterations in GABA shunt, TCA cycle, shikimate pathway, and metabolisms of lipids, amino acids, inositol, choline, pyrimidine, and purine. The hexose transporter has also been affected. These findings have shown that Tri5 gene deletion induces widespread changes in FG primary metabolism and demonstrated the combination of NMR-based metabonomics and qRT-PCR analyses as a useful way to understand the systems metabolic changes resulting from a single specific gene knockout in an eukaryotic genome and thus Tri5 gene functions.
学科主题	波谱分析
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	MULTIVARIATE DATA-ANALYSIS ; SPINNING H-1-NMR SPECTROSCOPY ; HEAD BLIGHT ; TRICHOTHECENE BIOSYNTHESIS ; ARABIDOPSIS-THALIANA ; REDUCED VIRULENCE ; WHEAT ; RESPONSES ; CHINA ; SCAB
收录类别	SCI
语种	英语
WOS记录号	WOS:000290234800013
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1789]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China 5.Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Chen, Fangfang,Zhang, Jingtao,Song, Xiushi,et al. Combined Metabonomic and Quantitative Real-Time PCR Analyses Reveal Systems Metabolic Changes of Fusarium graminearum Induced by Tri5 Gene Deletion[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(5):2273-2285.
APA	Chen, Fangfang.,Zhang, Jingtao.,Song, Xiushi.,Yang, Jian.,Li, Heping.,...&Liao, Yu-Cai.(2011).Combined Metabonomic and Quantitative Real-Time PCR Analyses Reveal Systems Metabolic Changes of Fusarium graminearum Induced by Tri5 Gene Deletion.JOURNAL OF PROTEOME RESEARCH,10(5),2273-2285.
MLA	Chen, Fangfang,et al."Combined Metabonomic and Quantitative Real-Time PCR Analyses Reveal Systems Metabolic Changes of Fusarium graminearum Induced by Tri5 Gene Deletion".JOURNAL OF PROTEOME RESEARCH 10.5(2011):2273-2285.