An efficient design strategy for a whole-cell biosensor based on engineered ribosome binding sequences

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	An efficient design strategy for a whole-cell biosensor based on engineered ribosome binding sequences
	Yu, Qing ; Li, Yan ; Ma, Anzhou ; Liu, Weifeng ; Wang, Hailin ; Zhuang, Guoqiang
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2011-11-01
卷号	401 期号:9 页码:2891—2898
中文摘要	In prokaryotes, the ribosome binding sequence (RBS), located in the 5' untranslated region (5' UTR) of an mRNA, plays a critical role in enhancing mRNA translation and stability. To evaluate the effect of the RBS on the sensitivity and signal intensity of an environmental whole-cell biosensor, three Escherichia coli-based biosensors that respond to benzene, toluene, ethylbenzene, and the xylenes (BTEX) were constructed; the three biosensors have the same Pu promoter and xylR regulator from the Pseudomonas putida TOL plasmid but differ in the engineered RBS in their reporter genes. The results from time and dose-dependent induction of luminescence activity by 2-chlorotoluene showed that the BTEX-SE and BTEX-SD biosensors with engineered RBS had signal intensities approximately 10-35 times higher than the primary BTEX-W biosensor. The limits of detection (LOD) of the BTEX-SE and BTEX-SD biosensors were also significantly lower than the LOD of the BTEX-W biosensor (20 +/- 5 mu mol L(-1) and 25 +/- 5 mu mol L(-1) vs. 120 +/- 10 mu mol L(-1)). Moreover, the BTEX-SE and BTEX-SD biosensors responded three times more rapidly to the analytes. These results suggest that rationally designed RBS in the 5' UTR of a reporter gene may be a promising strategy for increasing the sensitivity, signal intensity, and response speed of whole-cell biosensors.
公开日期	2012-10-15
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/6984]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Yu, Qing,Li, Yan,Ma, Anzhou,et al. An efficient design strategy for a whole-cell biosensor based on engineered ribosome binding sequences[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,401(9):2891—2898.
APA	Yu, Qing,Li, Yan,Ma, Anzhou,Liu, Weifeng,Wang, Hailin,&Zhuang, Guoqiang.(2011).An efficient design strategy for a whole-cell biosensor based on engineered ribosome binding sequences.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,401(9),2891—2898.
MLA	Yu, Qing,et al."An efficient design strategy for a whole-cell biosensor based on engineered ribosome binding sequences".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 401.9(2011):2891—2898.