Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber
	Wu, Mingyi 1; Xu, Li 1,2; Zhao, Longyan1,2 ; Xiao, Chuang1,2 ; Gao, Na 1,2; Luo, Lan 1; Yang, Lian 1; Li, Zi 1; Chen, Lingyun 3; Zhao, Jinhua1
刊名	MARINE DRUGS
	2015
卷号	13 期号:4 页码:2063-2084
英文摘要	Sulfated fucans, the complex polysaccharides, exhibit various biological activities. Herein, we purified two fucans from the sea cucumbers Holothuria edulis and Ludwigothurea grisea. Their structures were verified by means of HPGPC, FT-IR, GC-MS and NMR. As a result, a novel structural motif for this type of polymers is reported. The fucans have a unique structure composed of a central core of regular (1 -> 2) and (1 -> 3)-linked tetrasaccharide repeating units. Approximately 50% of the units from L. grisea (100% for H. edulis fucan) contain sides of oligosaccharides formed by nonsulfated fucose units linked to the O-4 position of the central core. Anticoagulant activity assays indicate that the sea cucumber fucans strongly inhibit human blood clotting through the intrinsic pathways of the coagulation cascade. Moreover, the mechanism of anticoagulant action of the fucans is selective inhibition of thrombin activity by heparin cofactor II. The distinctive tetrasaccharide repeating units contribute to the anticoagulant action. Additionally, unlike the fucans from marine alga, although the sea cucumber fucans have great molecular weights and affluent sulfates, they do not induce platelet aggregation. Overall, our results may be helpful in understanding the structure-function relationships of the well-defined polysaccharides from invertebrate as new types of safer anticoagulants.
类目[WOS]	Chemistry, Medicinal
研究领域[WOS]	Pharmacology & Pharmacy
关键词[WOS]	FUCOSYLATED CHONDROITIN SULFATE ; DEPOLYMERIZED HOLOTHURIAN GLYCOSAMINOGLYCAN ; STICHOPUS-JAPONICUS ; PHYSICOCHEMICAL CHARACTERISTICS ; OXIDATIVE-DEGRADATION ; BIOLOGICAL-PROPERTIES ; BLOOD PLATELETS ; HEPARIN ; POLYSACCHARIDES ; AGGREGATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000353715900024
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/20683]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China 2.Univ Chinese Acad Sci, Sch Life Sci, Beijing 100000, Peoples R China 3.Yunnan Univ TCM, Dept Pharm, Kunming 650200, Peoples R China
推荐引用方式 GB/T 7714	Wu, Mingyi,Xu, Li,Zhao, Longyan,et al. Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber[J]. MARINE DRUGS,2015,13(4):2063-2084.
APA	Wu, Mingyi.,Xu, Li.,Zhao, Longyan.,Xiao, Chuang.,Gao, Na.,...&Zhao, Jinhua.(2015).Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber.MARINE DRUGS,13(4),2063-2084.
MLA	Wu, Mingyi,et al."Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber".MARINE DRUGS 13.4(2015):2063-2084.