Dynamic Changes of Tyrosine Hydroxylase and Dopamine Concentrations in the Ventral Tegmental Area-Nucleus Accumbens Projection During the Expression of Morphine-Induced Conditioned Place Preference in Rats

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	Dynamic Changes of Tyrosine Hydroxylase and Dopamine Concentrations in the Ventral Tegmental Area-Nucleus Accumbens Projection During the Expression of Morphine-Induced Conditioned Place Preference in Rats
	Liang, Jing1,2,3 ; Ma, Si-Si 1,2; Li, Yi-Jing 1,2; Ping, Xing-Jie 1,2; Hu, Ling 1,2; Cui, Cai-Lian 1,2
刊名	NEUROCHEMICAL RESEARCH
	2012-07-01
卷号	37 期号:7 页码:1482-1489
关键词	Morphine Conditioned place preference (CPP) Dopamine (DA) Tyrosine hydroxylase (TH)
ISSN号	0364-3190
通讯作者	Cui, CL (reprint author), Peking Univ, Hlth Sci Ctr, Neurosci Res Inst, 38 Xueyuan Rd, Beijing 100083, Peoples R China.
产权排序	2
英文摘要	Our previous study demonstrated that morphine dose- and time-dependently elevated dopamine (DA) concentrations in the nucleus accumbens (NAc) during the expression of morphine-induced conditioned place preference (CPP) in rats. However, still unknown are how DA concentrations dynamically change during the morphine-induced CPP test and whether tyrosine hydroxylase (TH) activity in the ventral tegmental area (VTA) plays a vital role in this process. In the present study, we measured dynamic changes in TH and phosphorylated TH serine 40 (pTH Ser(40)) and pTH Ser(31) proteins in the VTA, and DA concentrations in the NAc at 5 min intervals during a 30 min morphine-induced CPP test. Rats that underwent morphine-induced CPP training significantly preferred the morphine-paired chamber during the CPP expression test, an effect that lasted at least 30 min in the drug-free state. DA concentrations in the NAc markedly increased at 15 min when the rats were returned to the CPP boxes to assess the expression of preference for the previously drug-paired chamber. DA concentrations then declined 2 h after the CPP test. TH and pTH Ser(40) levels, but not pTH Ser(31) levels, in the VTA were enhanced during the CPP test. These results indicated that TH and the phosphorylation of TH Ser(40) in the VTA may be responsible for DA synthesis and release in the NAc during the behavioral expression of conditioned reward elicited by a drug-associated context.
学科主题	Physiological psychology/Biological Psychology
收录类别	SCI
项目简介	This work was supported by grants from the National Natural Science Foundation (30970933) and National Basic Research Program (2009CB522003).
原文出处	http://nri.bjmu.edu.cn/english/html/2012publish/1204.pdf
语种	英语
WOS记录号	WOS:000304878200013
内容类型	期刊论文
源URL	[http://ir.psych.ac.cn/handle/311026/11477]
专题	心理研究所_中国科学院心理健康重点实验室
作者单位	1.Peking Univ, Hlth Sci Ctr, Neurosci Res Inst, Beijing 100083, Peoples R China 2.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Liang, Jing,Ma, Si-Si,Li, Yi-Jing,et al. Dynamic Changes of Tyrosine Hydroxylase and Dopamine Concentrations in the Ventral Tegmental Area-Nucleus Accumbens Projection During the Expression of Morphine-Induced Conditioned Place Preference in Rats[J]. NEUROCHEMICAL RESEARCH,2012,37(7):1482-1489.
APA	Liang, Jing,Ma, Si-Si,Li, Yi-Jing,Ping, Xing-Jie,Hu, Ling,&Cui, Cai-Lian.(2012).Dynamic Changes of Tyrosine Hydroxylase and Dopamine Concentrations in the Ventral Tegmental Area-Nucleus Accumbens Projection During the Expression of Morphine-Induced Conditioned Place Preference in Rats.NEUROCHEMICAL RESEARCH,37(7),1482-1489.
MLA	Liang, Jing,et al."Dynamic Changes of Tyrosine Hydroxylase and Dopamine Concentrations in the Ventral Tegmental Area-Nucleus Accumbens Projection During the Expression of Morphine-Induced Conditioned Place Preference in Rats".NEUROCHEMICAL RESEARCH 37.7(2012):1482-1489.