Structured illumination microscopy for super-resolution and optical sectioning

CORC > 西安光学精密机械研究所 > 中国科学院西安光学精密机械研究所 > 瞬态光学技术国家重点实验室

	Structured illumination microscopy for super-resolution and optical sectioning
	Dan, Dan; Yao, Baoli; Lei, Ming
刊名	chinese science bulletin
	2014-04-01
卷号	59 期号:12 页码:1291-1307
关键词	Microscopy Structured illumination Super-resolution Optical sectioning
ISSN号	1001-6538
英文摘要	optical microscopy plays an essential role in biological studies due to its capability and compatibility of non-contact, minimally invasive observation and measurement of live specimens. however, the conventional optical microscopy only has a spatial resolution about 200 nm due to the abbe diffraction limit, and also lacks the ability of three-dimensional imaging. super-resolution far-field optical microscopy based on special illumination schemes has been dramatically developed over the last decade. among them, only the structured illumination microscopy (sim) is of wide-field geometry that enables it easily compatible with the conventional optical microscope. in this article, the principle of sim was introduced in terms of point spread function (psf) and optical transform function (otf) of the optical system. the sim for super-resolution (sim-sr) proposed by gustafsson et al. and the sim for optical sectioning (sim-os) proposed by neil et al. are the most popular ones in the research community of microscopy. they have the same optical configuration, but with different data post-processing algorithms. we mathematically described the basic theories for both of the sims, respectively, and presented some numerical simulations to show the effects of super-resolution and optical sectioning. various approaches to generation of structured illumination patterns were reviewed. as an example, a sim system based on dmd-modulation and led-illumination was demonstrated. a lateral resolution of 90 nm was achieved with gold nano-particles. the optical sectioning capability of the microscope was demonstrated with golgi-stained mouse brain neurons, and the sectioning strength of 930 nm was obtained.
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	dynamic speckle illumination ; field fluorescence microscopy ; stimulated-emission-depletion ; stochastic transfer-function ; spatial light-modulator ; phase-shift estimation ; wide-field ; living cells ; full-field ; reconstruction microscopy
收录类别	SCI
语种	英语
WOS记录号	WOS:000333126800010
公开日期	2015-03-13
内容类型	期刊论文
源URL	[http://ir.opt.ac.cn/handle/181661/22179]
专题	西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位	Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China
推荐引用方式 GB/T 7714	Dan, Dan,Yao, Baoli,Lei, Ming. Structured illumination microscopy for super-resolution and optical sectioning[J]. chinese science bulletin,2014,59(12):1291-1307.
APA	Dan, Dan,Yao, Baoli,&Lei, Ming.(2014).Structured illumination microscopy for super-resolution and optical sectioning.chinese science bulletin,59(12),1291-1307.
MLA	Dan, Dan,et al."Structured illumination microscopy for super-resolution and optical sectioning".chinese science bulletin 59.12(2014):1291-1307.