Heterologous Expression and Efficient Secretion of Chitosanase from Microbacterium sp in Escherichia coli | |
Sun, Yuying1,2,3; Zhang, Jiquan2; Wang, Shujun1,3 | |
刊名 | INDIAN JOURNAL OF MICROBIOLOGY |
2015-06-01 | |
卷号 | 55期号:2页码:194-199 |
关键词 | Heterologous Secretion Microbacterium sp OU01 Recombinant expression vector Chitosanase |
英文摘要 | A recombinant expression vector, pCT7-CHISP6H, was constructed for the secretory expression of mature peptide of chitosanase (mMschito) from Microbacterium sp. OU01. The vector contains several elements, including T7 promoter, signal peptide sequence of mschito, 6 x His-tag sequence and PmaCI restriction enzyme cloning site. In pCT7-CHISP6H, mMschito was fused into signal peptide sequence of mschito gene to construct recombinant plasmid pCT7-CHISP6H-mMschito. The recombinant plasmid was transformed into Escherichia coli BL21(DE3) and then expressed. The recombinant protein was secreted into the Luria-Bertani broth and the chitosanase activity in supernatant of the culture could reach up to 67.56 U/mL. The rmMschito in the broth supernatant was purified using HisTrap (TM) FF Crude column and the purified rmMschito was shown to be apparent homogeneity by 12 % SDS-PAGE analysis. Detected by 4700 MALDI-TOF-TOF-MS, the molecular weight of the purified rmMschito was 26,758.1875 and it was consistent with the predicted molecular weight. Chitosan (degree of deacetylation of 99 %) was mostly hydrolyzed into chitopentaose, chitotriose, and chitobiose by the purified rmMschito. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000351468900009 |
公开日期 | 2015-06-16 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/25443] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Huaihai Inst Technol, Jiangsu Key Lab Marine Pharmaceut Compound Screen, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Huaihai Inst Technol, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, Yuying,Zhang, Jiquan,Wang, Shujun. Heterologous Expression and Efficient Secretion of Chitosanase from Microbacterium sp in Escherichia coli[J]. INDIAN JOURNAL OF MICROBIOLOGY,2015,55(2):194-199. |
APA | Sun, Yuying,Zhang, Jiquan,&Wang, Shujun.(2015).Heterologous Expression and Efficient Secretion of Chitosanase from Microbacterium sp in Escherichia coli.INDIAN JOURNAL OF MICROBIOLOGY,55(2),194-199. |
MLA | Sun, Yuying,et al."Heterologous Expression and Efficient Secretion of Chitosanase from Microbacterium sp in Escherichia coli".INDIAN JOURNAL OF MICROBIOLOGY 55.2(2015):194-199. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论