Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina | |
Sawaguchi, A. | |
刊名 | Journal of Histochemistry & Cytochemistry
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1999 | |
卷号 | 47期号:7页码:919-927 |
ISSN号 | 0022-1554 |
中文摘要 | We investigated the localization of polysialic acid (PSA), neural cell adhesion molecule (NCAM), and vesicular acetylcholine transporter (VAChT) in adult rat retina by using immunofluorescence with a confocal laser scanning microscope. Western blot analysis showed a typical broad smear of PSA and isoforms of NCAM (120, 140, and 180 kD). PSA immunofluorescence revealed multistratification in the inner plexiform layer (IPL). Dual immunostaining for PSA and NCAM exhibited the selective co-expression of PSA and NCAM on Muller cells. Moreover, dual immunolabeling for PSA and VAChT completely separated the five strata in the IPL. Strata 1, 3, and 5 were immunoreactive for PSA and Strata 2 and 4 for VAChT. These results suggest the possibility that PSA molecules on Muller cells are spatially related to ON and OFF retinal channels in the IPL. |
语种 | 英语 |
公开日期 | 2015-06-08 |
内容类型 | 期刊论文 |
源URL | [http://ir.xtbg.org.cn/handle/353005/8933] ![]() |
专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
推荐引用方式 GB/T 7714 | Sawaguchi, A.. Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina[J]. Journal of Histochemistry & Cytochemistry,1999,47(7):919-927. |
APA | Sawaguchi, A..(1999).Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina.Journal of Histochemistry & Cytochemistry,47(7),919-927. |
MLA | Sawaguchi, A.."Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina".Journal of Histochemistry & Cytochemistry 47.7(1999):919-927. |
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