Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina
	Sawaguchi, A.
刊名	Journal of Histochemistry & Cytochemistry
	1999
卷号	47 期号:7 页码:919-927
ISSN号	0022-1554
中文摘要	We investigated the localization of polysialic acid (PSA), neural cell adhesion molecule (NCAM), and vesicular acetylcholine transporter (VAChT) in adult rat retina by using immunofluorescence with a confocal laser scanning microscope. Western blot analysis showed a typical broad smear of PSA and isoforms of NCAM (120, 140, and 180 kD). PSA immunofluorescence revealed multistratification in the inner plexiform layer (IPL). Dual immunostaining for PSA and NCAM exhibited the selective co-expression of PSA and NCAM on Muller cells. Moreover, dual immunolabeling for PSA and VAChT completely separated the five strata in the IPL. Strata 1, 3, and 5 were immunoreactive for PSA and Strata 2 and 4 for VAChT. These results suggest the possibility that PSA molecules on Muller cells are spatially related to ON and OFF retinal channels in the IPL.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8933]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Sawaguchi, A.. Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina[J]. Journal of Histochemistry & Cytochemistry,1999,47(7):919-927.
APA	Sawaguchi, A..(1999).Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina.Journal of Histochemistry & Cytochemistry,47(7),919-927.
MLA	Sawaguchi, A.."Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina".Journal of Histochemistry & Cytochemistry 47.7(1999):919-927.