Dedifferentiating initiation and embryogenesis from freshly-isolated microspores of barley

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	Dedifferentiating initiation and embryogenesis from freshly-isolated microspores of barley
	Guo, X. R.
刊名	Science in China Series C-Life Sciences
	1997
卷号	40 期号:3 页码:332-+
ISSN号	1006-9305
中文摘要	By using DNA-specific fluorescent dye and a confocal laser scanning microscope, the present study was designed to investigate the cytological characteristics of dedifferentiating initiation during pretreatment and embryogenesis during culture in freshly-isolated microspores of barley, and the difference in main developmental pathway between freshly-isolated and cold-treated microspores; The results revealed that ( i) freshly-isolated microspores started the initiation within 12 h of mannitol pretreatment, whose main cytological characteristics were that: eel! volume was obviously extended; the volume of nuclei and nucleoli were also greatly increased; nucleoli were extremely clear and highly condensed; NIC ratio was very high; ( ii) all the pretreatment methods led to the initiation of the microspores, thus triggering the embryogenic process; ( iii) pretreatment methods influenced the main developmental pathway of microspores by changing the pattern of the first mitosis. The cold-treated microspores formed main developmental pathway via Et patterns, but freshly-isolated microspores via B pattern.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8869]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Guo, X. R.. Dedifferentiating initiation and embryogenesis from freshly-isolated microspores of barley[J]. Science in China Series C-Life Sciences,1997,40(3):332-+.
APA	Guo, X. R..(1997).Dedifferentiating initiation and embryogenesis from freshly-isolated microspores of barley.Science in China Series C-Life Sciences,40(3),332-+.
MLA	Guo, X. R.."Dedifferentiating initiation and embryogenesis from freshly-isolated microspores of barley".Science in China Series C-Life Sciences 40.3(1997):332-+.